Gargi College, University of Delhi, Siri Fort Road, New Delhi, 110 049, India.
Department of Zoology, Kirori Mal College, University of Delhi, Delhi, 110007, India.
Antonie Van Leeuwenhoek. 2023 Nov;116(11):1113-1121. doi: 10.1007/s10482-023-01871-8. Epub 2023 Aug 28.
The taxonomic status of 43 Psychrobacter species was examined based upon the genome sequences of their type strains. Three groups of type strains were found to be conspecific, Psychrobacter salsus Shivaji et al. (Syst Appl Microbiol 27:628-635, 2004. 10.1078/0723202042369956) and Psychrobacter submarinus Romanenko et al. (Int J Syst Evol Microbiol 52:1291-1297, 2002. 10.1099/00207713-52-4-1291); Psychrobacter oceani Matsuyama et al. (Int J Syst Evol Microbiol 65:1450-1455, 2015. 10.1099/ijs.0.000118) and Psychrobacter pacificensis Maruyama et al. (Int J Syst Evol Microbiol 50:835-846, 2000. 10.1099/00207713-50-2-835); and Psychrobacter proteolyticus Denner et al. (Syst Appl Microbiol 24:44-53, 2001. 10.1078/0723-2020-00006), Psychrobacter marincola Romanenko et al. (Int J Syst Evol Microbiol 52:1291-1297, 2002. 10.1099/00207713-52-4-1291) and Psychrobacter adeliensis Shivaji et al. (Syst Appl Microbiol 27:628-635, 2004. 10.1078/0723202042369956). For all three groups, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values are > 97.69% and > 80.2%, respectively. This conclusion is supported by similarities in morphology, growth properties, and fatty acid compositions. Based on this evidence, we propose the reclassification of Psychrobacter salsus Shivaji et al. (Syst Appl Microbiol 27:628-635, 2004. 10.1078/0723202042369956) as a later heterotypic synonym of Psychrobacter submarinus Romanenko et al. (Int J Syst Evol Microbiol 52:1291-1297, 2002. 10.1099/00207713-52-4-1291); Psychrobacter oceani Matsuyama et al. (Int J Syst Evol Microbiol 65:1450-1455, 2015. 10.1099/ijs.0.000118) as a later heterotypic synonym of Psychrobacter pacificensis Maruyama et al. (Int J Syst Evol Microbiol 50:835-846, 2000. 10.1099/00207713-50-2-835), and Psychrobacter marincola Romanenko et al. (Int J Syst Evol Microbiol 52:1291-1297, 2002. 10.1099/00207713-52-4-1291) and Psychrobacter adeliensis Shivaji et al. (Syst Appl Microbiol 27:628-635, 2004. 10.1078/0723202042369956) as later heterotypic synonyms of Psychrobacter proteolyticus Denner et al. (Syst Appl Microbiol 24:44-53, 2001. 10.1078/0723-2020-00006).
基于其模式菌株的基因组序列，对 43 种嗜冷菌的分类地位进行了研究。发现三组模式菌株为同种，分别为：沙吉氏盐单胞菌（Shivaji 等人，系统应用微生物学 27:628-635, 2004. 10.1078/0723202042369956）和深海盐单胞菌（Romanenko 等人，国际系统与进化微生物学杂志 52:1291-1297, 2002. 10.1099/00207713-52-4-1291）；海洋嗜冷杆菌（Matsuyama 等人，国际系统与进化微生物学杂志 65:1450-1455, 2015. 10.1099/ijs.0.000118）和太平洋嗜冷杆菌（Maruyama 等人，国际系统与进化微生物学杂志 50:835-846, 2000. 10.1099/00207713-50-2-835）；以及蛋白水解嗜冷杆菌（Denner 等人，系统应用微生物学 24:44-53, 2001. 10.1078/0723-2020-00006）、海洋栖冷杆菌（Romanenko 等人，国际系统与进化微生物学杂志 52:1291-1297, 2002. 10.1099/00207713-52-4-1291）和阿迪利氏嗜冷杆菌（Shivaji 等人，系统应用微生物学 27:628-635, 2004. 10.1078/0723202042369956）。对于这三组，平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为>97.69%和>80.2%。这一结论得到了形态学、生长特性和脂肪酸组成相似性的支持。基于这些证据，我们建议将沙吉氏盐单胞菌（Syst Appl Microbiol 27:628-635, 2004. 10.1078/0723202042369956）重新分类为深海盐单胞菌（Int J Syst Evol Microbiol 52:1291-1297, 2002. 10.1099/00207713-52-4-1291）的晚型异名；海洋栖冷杆菌（Int J Syst Evol Microbiol 65:1450-1455, 2015. 10.1099/ijs.0.000118）是太平洋嗜冷杆菌（Int J Syst Evol Microbiol 50:835-846, 2000. 10.1099/00207713-50-2-835）的晚型异名，而海洋栖冷杆菌（Romanenko 等人，国际系统与进化微生物学杂志 52:1291-1297, 2002. 10.1099/00207713-52-4-1291）和阿迪利氏嗜冷杆菌（Shivaji 等人，系统应用微生物学 27:628-635, 2004. 10.1078/0723202042369956）是蛋白水解嗜冷杆菌（Syst Appl Microbiol 24:44-53, 2001. 10.1078/0723-2020-00006）的晚型异名。
