East I J, Berrie D A
Res Vet Sci. 1986 Jul;41(1):129-30.
Maturation of Oesophagostomum radiatium is characterised by production of different proteins in all the life cycle stages that occur within the bovine host. One-dimensional sodium dodecyl sulphate-polyacrylamide gels stained for total protein, showed that fourth stage larvae (L4) and adult worms have many proteins in common but L4 has a major additional protein (82,000 D); third stage larvae have many different proteins. Analysis of the proteins of each life cycle stage by Western blotting with sera from naturally infected calves showed that each life cycle stage had specific antigenic proteins. The major protein unique to L4 was identified as a developmentally regulated antigen.
辐射食道口线虫的成熟特征是在牛宿主体内发生的所有生命周期阶段产生不同的蛋白质。用考马斯亮蓝染色的一维十二烷基硫酸钠-聚丙烯酰胺凝胶显示，第四期幼虫（L4）和成虫有许多共同的蛋白质，但L4有一个主要的额外蛋白质（82,000 D）；第三期幼虫有许多不同的蛋白质。用自然感染小牛的血清进行蛋白质免疫印迹分析，结果表明每个生命周期阶段都有特定的抗原蛋白。L4特有的主要蛋白质被鉴定为一种发育调控抗原。
