Afzal M, Tengerdy R P, Brodie S J, Demartini J C, Ellis R P, Jones R L, Kimberling C V
Res Vet Sci. 1986 Jul;41(1):85-9.
Cellular as well as humoral immune responses were detected in six rams experimentally infected with Brucella ovis. Specific antibodies were detectable by enzyme-linked immunosorbent assay by day 11 after infection in all the rams. The levels of IgM antibodies and total antibodies in the serum rose until 33 and 41 days after infection respectively, then levelled off. Antigen-induced blastogenic responses by lymphocytes developed as early as five days after infection in all rams but had decreased to low levels by day 63 in most. Blastogenesis induced by phytohaemagglutinin and concanavalin A varied among infected rams and did not differ significantly (P greater than 0.05) from control rams. All rams had developed delayed-type skin hypersensitivity by day 63 after infection. One ram which did not become infected as a result of exposure had low levels of B ovis serum antibodies and a detectable antigen-induced lymphocyte blastogenic response before infection, suggesting the involvement of cell-mediated immunity in protection against B ovis.
在六只经实验感染绵羊布鲁氏菌的公羊中检测到了细胞免疫和体液免疫反应。在感染后第11天，通过酶联免疫吸附测定法在所有公羊中均可检测到特异性抗体。血清中IgM抗体和总抗体水平分别在感染后33天和41天之前上升，然后趋于平稳。抗原诱导的淋巴细胞增殖反应在所有公羊感染后最早5天出现，但在大多数公羊中到第63天时已降至低水平。感染公羊中由植物血凝素和刀豆球蛋白A诱导的增殖反应各不相同，与对照公羊相比无显著差异（P大于0.05）。所有公羊在感染后第63天时均出现了迟发型皮肤超敏反应。一只因接触而未被感染的公羊在感染前血清中绵羊布鲁氏菌抗体水平较低，且有可检测到的抗原诱导的淋巴细胞增殖反应，这表明细胞介导的免疫参与了对绵羊布鲁氏菌的保护作用。
