Fensterbank R, Pardon P, Marly J
Ann Rech Vet. 1982;13(2):185-90.
The efficacy of Brucella melitensis strain Rev. 1 vaccine against a Brucella ovis experimental infection was judged on 30 rams, 15 of which had received 8.5 X 10(8) Rev. 1 at the age of 4 months. All were challenged 9 months later by conjunctival route with 5 X 10(8) B. ovis. The 30 rams were killed three months after challenge and were autopsied. Genital and other organs and lymph-nodes were searched for B. ovis. Only a few organs and lymph-nodes of three vaccinated rams shed B. ovis, contrary to controls which were all heavily infected. The distribution of infection indicated that the most frequently infected organs are the vesicular glands which, more than epididymes, seem to be the target organs of the infection. These results show that immunization with strain Rev. 1 is very effective against B. ovis infection. It is recommended to vaccinate rams of four to six months in an area where B. ovis or B. melitensis infection, or both, are prevalent.
用羊种布鲁氏菌Rev. 1疫苗对30只公羊进行免疫，以评估其对绵羊布鲁氏菌实验性感染的效力。其中15只在4月龄时接种8.5×10⁸个Rev. 1菌苗。9个月后，所有公羊均经结膜途径接种5×10⁸个绵羊布鲁氏菌进行攻毒。攻毒3个月后，将30只公羊宰杀并进行尸检。检查生殖器官、其他器官及淋巴结，查找绵羊布鲁氏菌。与全部严重感染的对照公羊不同，仅3只接种疫苗的公羊的少数器官和淋巴结排出绵羊布鲁氏菌。感染分布情况表明，最常受感染的器官是精囊腺，与附睾相比，精囊腺似乎是感染的靶器官。这些结果表明，用Rev. 1菌株免疫对绵羊布鲁氏菌感染非常有效。建议在绵羊布鲁氏菌或羊种布鲁氏菌感染或两者均流行的地区，对4至6月龄的公羊进行疫苗接种。
