Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Guangdong, China.
Department of Obstetrics and Gynecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
J Cell Physiol. 2023 Nov;238(11):2535-2545. doi: 10.1002/jcp.31102. Epub 2023 Aug 29.
During the oocyte growth, maturation and zygote development, chromatin structure keeps changing to regulate different nuclear activities. Here, we reported the role of SMC2, a core component of condensin complex, in oocyte and embryo development. Oocyte-specific conditional knockout of SMC2 caused female infertility. In the absence of SMC2, oocyte meiotic maturation and ovulation occurred normally, but chromosome condensation showed defects and DNA damages were accumulated in oocytes. The pronuclei were abnormally organized and micronuclei were frequently observed in fertilized eggs, their activity was impaired, and embryo development was arrested at the one-cell stage, suggesting that maternal SMC2 is essential for embryonic development.
在卵母细胞生长、成熟和受精卵发育过程中，染色质结构不断变化以调节不同的核活动。在这里，我们报道了SMC2（凝聚素复合物的核心组成部分）在卵母细胞和胚胎发育中的作用。卵母细胞特异性条件性敲除 SMC2 导致雌性不孕。在缺乏 SMC2 的情况下，卵母细胞减数分裂成熟和排卵正常发生，但染色体浓缩出现缺陷，卵母细胞中积累了 DNA 损伤。原核异常排列，受精卵中经常观察到微核，其活性受损，胚胎发育停滞在单细胞阶段，表明母源 SMC2 对胚胎发育至关重要。
