Narra Sai Sandhya, Gence Laura, Youssouf Latufa, Couprie Joël, Giraud Pierre, Diotel Nicolas, Lefebvre D'Hellencourt Christian
Université de La Réunion, INSERM, UMR 1188, Diabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), Saint-Denis, La Réunion, France.
Zebrafish. 2023 Oct;20(5):200-209. doi: 10.1089/zeb.2023.0007. Epub 2023 Aug 29.
Regenerative medicine is an emerging field of research aiming to understand the wound healing mechanisms and to develop new therapeutic strategies. Nanocarriers are used to improve drug bioavailability, solubility, and therapeutic abilities. In this study, we used for the first time curcumin loaded oligo kappa-carrageenan-graft-polycaprolactone (oligoKC-g-PCL) nanomicelles to investigate their regenerative potential using a model of tail amputation in zebrafish eleutheroembryo. First, we showed that curcumin encapsulated oligoKC-g-PCL spherical micelles had a mean size of 92 ± 32 nm and that micelles were successfully loaded with curcumin. These micelles showed a slow and controlled drug release over 72 h. The toxicity of curcumin nanomicelles was then tested on zebrafish eleutheroembryo based on the survival rate after 24 h. At nontoxic concentration, curcumin nanomicelles improved tail regeneration within 3 days postamputation, compared with empty micelles or curcumin alone. Furthermore, we demonstrated that curcumin nanomicelles increased the recruitment of neutrophils and macrophages 6 h postlesion. Finally, our study highlights the efficiency of oligoKC-g-PCL nanomicelles for encapsulation of hydrophobic molecules such as curcumin. Indeed, our study demonstrates that curcumin nanomicelles can modulate inflammatory reactions and promote regenerative processes. However, further investigations will be required to better understand the mechanisms sustaining regeneration and to develop new therapeutics.
再生医学是一个新兴的研究领域，旨在了解伤口愈合机制并开发新的治疗策略。纳米载体用于提高药物的生物利用度、溶解度和治疗能力。在本研究中，我们首次使用负载姜黄素的低聚κ-卡拉胶接枝聚己内酯（oligoKC-g-PCL）纳米胶束，利用斑马鱼幼胚尾部截肢模型研究其再生潜力。首先，我们表明包封姜黄素的oligoKC-g-PCL球形胶束的平均尺寸为92±32nm，并且胶束成功负载了姜黄素。这些胶束在72小时内显示出缓慢且可控的药物释放。然后根据24小时后的存活率，在斑马鱼幼胚上测试姜黄素纳米胶束 的毒性。在无毒浓度下，与空胶束或单独的姜黄素相比，姜黄素纳米胶束在截肢后3天内改善了尾部再生。此外，我们证明姜黄素纳米胶束在损伤后6小时增加了中性粒细胞和巨噬细胞的募集。最后，我们的研究突出了oligoKC-g-PCL纳米胶束对封装姜黄素等疏水分子的效率。事实上，我们的研究表明姜黄素纳米胶束可以调节炎症反应并促进再生过程。然而，需要进一步研究以更好地理解维持再生的机制并开发新的治疗方法。
