Weng Zongkuan, Deng Yitong, Tang Fen, Zhao Lukuan, Zhao Lingxiao, Wang Yuan, Dai Xibin, Zhou Zhilin, Cao Qinghe
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Institute of Sweetpotato Research, Chinese Academy of Agricultural Sciences, Xuzhou, 221121, China.
Plant Methods. 2023 Aug 29;19(1):93. doi: 10.1186/s13007-023-01050-w.
Sweetpotato is an important vegetable and food crop that is bred through sexual crosses and systematic selection. The use of in vitro germination of sweetpotato pollen to test its viability has important theoretical and practical implications for improving the efficiency of sweetpotato crossbreeding by controlling pollination and conducting research on sweetpotato pollen biology.
In this study, we observed the morphological structure of sweetpotato pollen under a scanning electron microscope (SEM), developed an effective method for the in vitro germination of sweetpotato pollen, and examined the viability of sweetpotato pollen after treating plants at different temperatures before blossoming. Sweetpotato pollen grains are spherical, with an average diameter of 87.07 ± 3.27 μm (excluding spines), with multiple germination pores and reticulate pollen surface sculpture. We applied numerous media to sweetpotato pollen germination in vitro to screen the initial medium and optimised the medium components through single-factor design. The most effective liquid medium for in vitro sweetpotato pollen germination contained 50 g/L Sucrose, 50 g/L Polyethylene glycol 4000 (PEG4000), 100 mg/L Boric acid and 300 mg/L Calcium nitrate, with a pH = 6.0. The optimum growth temperature for pollen development in sweetpotato was from 25 to 30 °C. Neither staining nor in situ germination could accurately determine the viability of sweetpotato pollen.
In vitro germination can be used to effectively determine sweetpotato pollen viability. The best liquid medium for in vitro germination of sweetpotato pollen contained 50 g/L Sucrose, 50 g/L Polyethylene glycol 4000 (PEG4000), 100 mg/L Boric acid and 300 mg/L Calcium nitrate, with the pH adjusted to 6.0. This study provides a reliable medium for the detection of sweetpotato pollen viability, which can provide a theoretical reference for sweetpotato genetics and breeding.
甘薯是一种重要的蔬菜和粮食作物，通过有性杂交和系统选育进行培育。利用甘薯花粉的离体萌发来检测其活力，对于通过控制授粉提高甘薯杂交育种效率以及开展甘薯花粉生物学研究具有重要的理论和实际意义。
在本研究中，我们在扫描电子显微镜（SEM）下观察了甘薯花粉的形态结构，开发了一种有效的甘薯花粉离体萌发方法，并检测了开花前在不同温度下处理植株后甘薯花粉的活力。甘薯花粉粒呈球形，平均直径为87.07±3.27μm（不包括刺），有多个萌发孔，花粉表面为网状纹饰。我们将多种培养基应用于甘薯花粉的离体萌发，以筛选初始培养基，并通过单因素设计优化培养基成分。用于甘薯花粉离体萌发的最有效液体培养基含有50g/L蔗糖、50g/L聚乙二醇4000（PEG4000）、100mg/L硼酸和300mg/L硝酸钙，pH值为6.0。甘薯花粉发育的最佳生长温度为25至30°C。染色法和原位萌发法均不能准确测定甘薯花粉的活力。
离体萌发可有效测定甘薯花粉活力。用于甘薯花粉离体萌发的最佳液体培养基含有50g/L蔗糖、50g/L聚乙二醇4000（PEG4000）、100mg/L硼酸和300mg/L硝酸钙，pH值调至6.0。本研究为检测甘薯花粉活力提供了一种可靠的培养基，可为甘薯遗传育种提供理论参考。
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