银杏叶提取物通过瞬时受体电位香草酸亚型1通道在体外阿尔茨海默病模型中的保护和治疗作用
Protective and Therapeutic Role of Ginkgo Biloba Extract Through TRPV1 Channels in an in Vitro Alzheimer's Disease Model.
作者信息
Özşimşek Ahmet, Övey İshak Suat, Karaçay Ertan, Yuluğ Burak
机构信息
Department of Neurology, Medical School of Alanya Alaaddin Keykubat University, Turkey.
Department of Physiology, Medical School of Alanya Alaaddin Keykubat University, Turkey.
出版信息
Noro Psikiyatr Ars. 2023 Aug 5;60(3):207-213. doi: 10.29399/npa.28166. eCollection 2023.
INTRODUCTION
The effect of Ginkgo biloba (GB) on mitochondria-dependent TRPV1 ion channels in neuroblastoma cells was investigated by creating an Alzheimer's disease (AD) model.
METHODS
Okadaic acid was applied on SH-SY5Y cells to create an AD model. After cellular differentiation, the study was organized with the seven main groups, examining the effect of GB on calcium depended TRPV1 channels in neuroblastoma cells AD, has been established in vitro.
RESULTS
The higher Ca+ concentration was detected in the GB+AD, AD and AD+GB groups when compared with the control (p<0.001). The Ca+ level was lower in GB+AD and AD+GB groups than in the AD group (p<0.001). Also, cytosolic Ca+ concentration was lower in the GB+AD than in the AD+GB group (p<0.05), the apoptosis and intracellular reactive oxygen species (ROS) values were higher in the GB+AD, AD and AD+GB groups than in the control (p<0.001). The apoptosis and intracellular ROS values were higher in AD group than in the GB+AD and AD+GB group (p<0.001) and the apoptosis level was higher in AD+GB group than GB+AD group (p<0.001) and the mitochondrial depolarization, caspase 3 and caspase 9 levels were higher in the GB+AD, AD and AD+GB groups when compared to the control group (p<0.001). Also, the values were lower in the GB+AD group, AD group and AD+GB groups when compared with the GB+AD+capsazepine group, AD+capsazepine group and AD+GB+capsazepine respectively (p<0.001).
CONCLUSION
These results show us that GB has a protective effect besides its therapeutic effect in Alzheimer's disease via TRPV1 channel.
引言
通过建立阿尔茨海默病（AD）模型，研究银杏叶（GB）对神经母细胞瘤细胞中线粒体依赖性TRPV1离子通道的影响。
方法
将冈田酸应用于SH-SY5Y细胞以建立AD模型。细胞分化后，将研究分为七个主要组，检测GB对体外建立的AD神经母细胞瘤细胞中钙依赖性TRPV1通道的影响。
结果
与对照组相比，GB+AD组、AD组和AD+GB组检测到更高的Ca+浓度（p<0.001）。GB+AD组和AD+GB组的Ca+水平低于AD组（p<0.001）。此外，GB+AD组的胞质Ca+浓度低于AD+GB组（p<0.05），GB+AD组、AD组和AD+GB组的凋亡和细胞内活性氧（ROS）值高于对照组（p<0.001）。AD组的凋亡和细胞内ROS值高于GB+AD组和AD+GB组（p<0.001），AD+GB组的凋亡水平高于GB+AD组（p<0.001），与对照组相比，GB+AD组、AD组和AD+GB组的线粒体去极化、半胱天冬酶3和半胱天冬酶9水平更高（p<0.001）。此外，与GB+AD+辣椒素组、AD+辣椒素组和AD+GB+辣椒素组相比，GB+AD组、AD组和AD+GB组的值分别更低（p<0.001）。
结论
这些结果表明，GB在阿尔茨海默病中除了具有治疗作用外，还通过TRPV1通道发挥保护作用。
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