Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Food Funct. 2023 Sep 19;14(18):8467-8486. doi: 10.1039/d3fo01408h.
The objective of this study was to evaluate germinated chickpea protein hydrolysate (GCPH) for its effect on markers of type 2 diabetes (T2D) and bitter taste receptor expression in intestinal epithelial cells. Protein hydrolysate was obtained using ficin, and the resulting peptides were sequenced using LC-ESI-MS/MS. Caco-2 cells were used to determine glucose uptake and extra-oral bitter receptor activation. Three peptides, VVFW, GEAGR, and FDLPAL, were identified in legumin. FDLPAL was the most potent peptide in molecular docking studies with a DPP-IV energy of affinity of -9.8 kcal mol. GCPH significantly inhibited DPP-IV production by Caco-2 cells (IC = 2.1 mM). Glucose uptake was inhibited in a dose-dependent manner (IC = 2.0 mM). A negative correlation was found between glucose uptake and PLCβ2 expression in Caco-2 cells ( value, -0.62). Thus, GCPH has the potential to be commercialized as a functional ingredient.
本研究旨在评估发芽鹰嘴豆蛋白水解物（GCPH）对 2 型糖尿病（T2D）标志物和肠道上皮细胞中苦味受体表达的影响。使用ficin 获得蛋白水解物，并用 LC-ESI-MS/MS 对所得肽进行测序。Caco-2 细胞用于测定葡萄糖摄取和口腔外苦味受体激活。在legumin 中鉴定出三种肽，VVFW、GEAGR 和 FDLPAL。FDLPAL 是与 DPP-IV 亲和力能量为-9.8 kcal/mol 的分子对接研究中最有效的肽。GCPH 可显著抑制 Caco-2 细胞中 DPP-IV 的产生（IC=2.1 mM）。葡萄糖摄取呈剂量依赖性抑制（IC=2.0 mM）。在 Caco-2 细胞中，葡萄糖摄取与 PLCβ2 表达呈负相关（ 值，-0.62）。因此，GCPH 有可能作为功能性成分进行商业化。
