African Genome Center, Mohammed VI Polytechnic University, Ben Guerir, Morocco.
Methods Mol Biol. 2023;2703:45-57. doi: 10.1007/978-1-0716-3389-2_4.
Transposon elements (TEs) are mobile genetic elements that can insert themselves into new locations and modify the plant genome. In recent years, they have been used as molecular markers in plant breeding programs. TE-based molecular markers (TE-markers) are divided into two categories depending on the transcription mechanism of the TEs. The first category is retrotransposon-based molecular markers, which include RBIP, IRAP, REMAP, and iPBS. The second group is DNA-based-TE-markers, which include MITE, TE-junction, and CACTA TE-markers. These markers are a good tool for studying genetic diversity and can provide information on plants' phylogenetic and evolutionary history. They can help improve breeding programs to increase agronomic traits and develop new varieties. Overall, TE-markers play an important role in plant genetics and plant breeding and contribute to a better understanding of plant biology. Here, we present TEMM, a curated data resource for TE-markers in plants. Relevant research articles were screened to collect primer sequences and related information. Only articles containing primer sequences are added to the present data resource. TEMM contains 784 primers with their associated PCR reaction programs and their applications in various crops. These include 203 IPBS, 191 RBIP, 140 IRAP, 78 TE-junction, 76 IRAPS, 47 RBIP-IRAP, 16 IRAP-REMAP, 12 REMAP, 12 REMA-IRAP, 6 REMA, and 3 ISBP primers. The data resource is freely available at https://bioinformatics.um6p.ma/TEMM .
转座子元件(TEs)是可插入新位置并改变植物基因组的移动遗传元件。近年来,它们已被用作植物育种计划中的分子标记。基于 TE 的分子标记(TE 标记)根据 TEs 的转录机制分为两类。第一类是基于反转录转座子的分子标记,包括 RBIP、IRAP、REMAP 和 iPBS。第二类是基于 DNA 的 TE 标记,包括 MITE、TE 接头和 CACTA TE 标记。这些标记是研究遗传多样性的良好工具,可以提供有关植物系统发育和进化历史的信息。它们有助于改善育种计划,增加农艺性状并开发新品种。总的来说,TE 标记在植物遗传学和植物育种中发挥着重要作用,有助于更好地理解植物生物学。在这里,我们介绍 TEMM,这是一个植物 TE 标记的精心策划的数据资源。筛选了相关研究文章以收集引物序列和相关信息。只有包含引物序列的文章才会添加到本数据资源中。TEMM 包含 784 个引物及其相关 PCR 反应程序,以及它们在各种作物中的应用。其中包括 203 个 iPBS、191 个 RBIP、140 个 IRAP、78 个 TE 接头、76 个 IRAPS、47 个 RBIP-IRAP、16 个 IRAP-REMAP、12 个 REMAP、12 个 REMA-IRAP、6 个 REMA 和 3 个 ISBP 引物。该数据资源可在 https://bioinformatics.um6p.ma/TEMM 上免费获取。