Jiangyin Hospital Affiliated to Nanjing University of Chinese Medicine, Jiangyin 214400, China.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
J Chromatogr Sci. 2024 Apr 23;62(4):321-327. doi: 10.1093/chromsci/bmad074.
Pinus massoniana needles, a traditional herb, were applied to prevent hair loss in China. Studies available mainly focused on pine needle flavonoids with various biological activities. However, there has been no pharmacokinetics study of the flavonoids from Pinus needles extract. A selective and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously quantify taxifolin, quercetin and catechin in rat plasma. To separate the three constituents, an Agilent Extend-C18 column (2.1 mm × 100 mm, 1.8 μm) was used with a mobile phrase of (A) 0.1% formic acid and (B) acetonitrile. The analytes were measured by multiple reaction monitoring in the negative ionization mode. There was good linearity in the established UHPLC-MS/MS method, with a coefficient of determination (r2) of >0.99. The accuracy, intra-day and inter-day precision and recovery were all satisfactory and these 3 compounds were stable under the tested conditions. The validated method in this study was successfully applied to pharmacokinetic study in healthy rats after oral and transdermal administration of Pinus needles extract. The results could provide further research foundation for pine needle extract as external preparations.
马尾松松针是一种传统草药，在中国被用于防止脱发。现有研究主要集中在具有多种生物活性的松针类黄酮上。然而，目前还没有马尾松针提取物中类黄酮的药代动力学研究。本研究建立了一种选择性和灵敏的超高效液相色谱-串联质谱（UHPLC-MS/MS）方法，用于同时定量测定大鼠血浆中的杨梅素、槲皮素和儿茶素。为了分离这三种成分，采用了 Agilent Extend-C18 柱（2.1mm×100mm，1.8μm），以（A）0.1%甲酸和（B）乙腈作为流动相。采用负离子多反应监测模式进行分析。该 UHPLC-MS/MS 方法具有良好的线性，相关系数（r2）均大于 0.99。该方法的准确度、日内和日间精密度及回收率均令人满意，并且这 3 种化合物在测试条件下均稳定。本研究建立的方法成功应用于马尾松针提取物经口和经皮给药后的健康大鼠的药代动力学研究。该研究结果可为马尾松针提取物作为外用制剂提供进一步的研究基础。
