Kaibara M, Ukiya Y, Sekiguchi M
Thromb Res. 1986 Aug 15;43(4):395-408. doi: 10.1016/0049-3848(86)90084-8.
Effects of mechanical trauma of platelets and red blood cells on dynamic viscoelasticity of blood during clotting were examined. Two different methods were attempted to give the mechanical damage to blood cells. In one method, a flow apparatus consisting of a thin teflon tube and two sample reservoirs connecting to both ends of the tube was employed. After a blood sample repeated the shuttle flow many times through the tube, the dynamic rigidity modulus (G') and loss modulus (G") of the blood sample during clotting were measured. The mechanical trauma of platelets brought about the increase of G' and G" for clots of PRP and whole blood, in addition to shorter clotting time. Slight hemolysis of red blood cells (less than 0.5% of total hemolysis) only yielded shorter clotting time for whole blood. In other method, packed red blood cells were hemolyzed by exposing the ultrasonic wave (the amount of hemolysis was about 5% of total hemolysis). Addition of supernatant of hemolyzed red blood cells to PRP caused the platelet aggregation. Furthermore, a marked hemolysis brought about higher values of G' and G" for blood clot, in addition to shorter clotting time. These results suggest that the release of constituents from damaged red blood cells and alteration of cell surface due to mechanical damage would accelerate the coagulation sequence.
研究了血小板和红细胞的机械损伤对血液凝固过程中动态粘弹性的影响。尝试了两种不同的方法对血细胞造成机械损伤。一种方法是使用由细聚四氟乙烯管和连接到管两端的两个样品储存器组成的流动装置。血液样本通过该管反复穿梭流动多次后，测量血液样本在凝血过程中的动态刚性模量（G'）和损耗模量（G"）。血小板的机械损伤导致富血小板血浆（PRP）和全血凝块的G'和G"增加，凝血时间也缩短。红细胞的轻微溶血（总溶血率小于0.5%）仅使全血的凝血时间缩短。在另一种方法中，通过暴露超声波使压积红细胞溶血（溶血程度约为总溶血率的5%）。将溶血红细胞的上清液添加到PRP中会导致血小板聚集。此外，明显的溶血除了使凝血时间缩短外，还导致血凝块的G'和G"值升高。这些结果表明，受损红细胞成分的释放以及机械损伤引起的细胞表面改变会加速凝血过程。
