Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan;
Anticancer Res. 2023 Sep;43(9):3913-3921. doi: 10.21873/anticanres.16579.
BACKGROUND/AIM: F-fluoro-deoxy-glucose positron emission tomography (F-FDG PET) has become indispensable for staging colorectal cancer but has limitations. Thus, PET with a focus on metabolism other than glucose, mainly amino acid metabolism, has been developed. L-type amino acid transporter 1 (LAT1) is known to be a cancer-specific amino acid transporter and, although 4-Borono-2-(18)F-fluoro-phenylalanine (FBPA) has been reported to be useful as a probe for LAT1, the significance of LAT1 expression in colorectal cancer is ambiguous and implementation of F-FBPA-PET in colorectal cancer has not yet been reported.
The aims of this study were to investigate the expression of LAT1 in primary lesions and metastatic lesions of colorectal cancer by immunohistochemical analysis and report the initial experience of performing F-FBPA-PET on colorectal cancer patients in clinical practice.
There was a significant correlation between LAT1 protein expression in primary tumors and liver metastases. Furthermore LAT-1 expression was positively correlated with recurrence (p=0.033). We performed F-FBPA-PET on three rectal cancer patients and detected cancer.
LAT1 protein is expressed not only in the primary colorectal tumor, but also in liver metastases. F-FBPA-PET can be safely performed in patients with colorectal cancer.
背景/目的：氟代脱氧葡萄糖正电子发射断层扫描（F-FDG PET）在结直肠癌分期中已不可或缺，但存在局限性。因此，已开发出针对葡萄糖以外的代谢物，主要是氨基酸代谢的 PET。已知 L 型氨基酸转运蛋白 1（LAT1）是一种癌症特异性氨基酸转运蛋白，虽然 4-硼代-2-（18）F-氟代苯丙氨酸（FBPA）已被报道可用作 LAT1 的探针，但 LAT1 在结直肠癌中的表达意义尚不清楚，并且 F-FBPA-PET 在结直肠癌中的应用尚未报道。
本研究的目的是通过免疫组织化学分析研究 LAT1 在结直肠癌原发灶和转移灶中的表达，并报告在临床实践中对结直肠癌患者进行 F-FBPA-PET 的初步经验。
原发肿瘤和肝转移瘤中 LAT1 蛋白表达存在显著相关性。此外，LAT-1 表达与复发呈正相关（p=0.033）。我们对 3 例直肠癌患者进行了 F-FBPA-PET 检查，检测到了癌症。
LAT1 蛋白不仅在结直肠原发肿瘤中表达，也在肝转移瘤中表达。F-FBPA-PET 可在结直肠癌患者中安全进行。
