School of Petrochemical Engineering, Changzhou University, ChangzhouJiangsu, 213164, China.
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, 213164, Jiangsu, China.
Mikrochim Acta. 2023 Aug 30;190(9):373. doi: 10.1007/s00604-023-05956-z.
Ciprofloxacin (CIP), a quinolone antibiotic, was rapidly and sensitively detected by integrating the molecularly imprinted polymer (MIP) with an ultra-sensitive electrochemiluminescence (ECL) method. g-CN, a typical polymer semiconductor, exhibited outstanding ECL efficiency and excellent ECL stability after combining with an iron-based metal-organic framework (MIL-101). Subsequently, the molecularly imprinted polypyrrole was electropolymerized on the composites of MIL-101-g-CN modified glassy carbon electrode (GCE). The specific sites that could target rebinding the CIP molecules were formed on the surface of MIP after extracting the CIP templates. The determination of specific concentrations of CIP could be realized according to the difference in ECL intensity (△ECL) between the eluting and rebinding of the CIP. Under optimal conditions, a good linear response of △ECL and the logarithm of CIP concentrations was obtained in the range 1.0 × 10 ~ 1.0 × 10 mol/L, with a detection limit of 4.5 × 10 mol/L (S/N = 3) (the working potential was -1.8 ~ 0 V). The RSD of all points in the calibration plot was less than 5.0% and the real samples recovery was between 98.0 and 104%. This paper displays satisfactory selectivity and sensitivity, providing a rapid, convenient, and cheap method for the determination of CIP in real samples.
环丙沙星(CIP)是一种喹诺酮类抗生素,通过将分子印迹聚合物(MIP)与超灵敏的电化学发光(ECL)方法相结合,可以快速、灵敏地检测到。g-CN 是一种典型的聚合物半导体,与基于铁的金属-有机骨架(MIL-101)结合后表现出出色的 ECL 效率和优异的 ECL 稳定性。随后,在 MIL-101-g-CN 修饰的玻碳电极(GCE)复合材料上电聚合分子印迹聚吡咯。在提取 CIP 模板后,MIP 表面形成了可以靶向结合 CIP 分子的特定结合位点。根据 CIP 洗脱和结合的 ECL 强度(△ECL)差异,可以实现对特定浓度 CIP 的测定。在最佳条件下,△ECL 与 CIP 浓度的对数在 1.0×101.0×10 mol/L 范围内呈现良好的线性响应,检测限为 4.5×10 mol/L(S/N=3)(工作电位为-1.80 V)。校准图中所有点的 RSD 均小于 5.0%,实际样品的回收率在 98.0%至 104%之间。本文显示出令人满意的选择性和灵敏度,为实际样品中 CIP 的测定提供了一种快速、方便、廉价的方法。