Masukawa H, Kaneko M, Takahashi T, Ishikawa M, Suzuki Y
Tohoku J Exp Med. 1986 Jul;149(3):241-9. doi: 10.1620/tjem.149.241.
The effects of intraluminal theophylline or prostaglandin E1 (PGE1) on transepithelial potential difference (PD) were examined in the human jejunum in vivo with a quadruple lumen perfusion tube. To measure transepithelial PDs, we placed one of the Ag/AgCl electrodes in the jejunal lumen and the other in the subcutaneous region of the left forearm of the subjects. Infusion of theophylline or PGE1 into the jejunal lumen produced a change in the PD, the lumen being more negatively charged. The magnitude of the change in PD for 2.4 X 10(-3) M theophylline was 3.3 +/- 0.48 mV (mean +/- S.E., n = 6) and that for 10(-6) M PGE1 was 5.0 +/- 0.60 mV (n = 6). In addition, the change in the PD induced by simultaneous infusion of both 2.4 X 10(-3) M theophylline and 10(-7) M PGE1 was about twice as large as the sum of those induced by individual infusion of the same concentration of theophylline or PGE1. We concluded that these PD changes occurred primarily by stimulation of electrogenic Cl secretion in the jejunum. Further exploitation of this method to determine PD might be useful for clinical investigation of the intestinal secretion.
使用四腔灌注管在人体空肠内检测腔内注射氨茶碱或前列腺素E1（PGE1）对跨上皮电位差（PD）的影响。为测量跨上皮PD，我们将其中一个Ag/AgCl电极置于空肠腔内，另一个置于受试者左前臂的皮下区域。向空肠腔内注入氨茶碱或PGE1会使PD发生变化，管腔带更多负电荷。2.4×10（-3）M氨茶碱引起的PD变化幅度为3.3±0.48mV（平均值±标准误，n = 6），10（-6）M PGE1引起的PD变化幅度为5.0±0.60mV（n = 6）。此外，同时注入2.4×10（-3）M氨茶碱和10（-7）M PGE1所诱导的PD变化约为单独注入相同浓度氨茶碱或PGE1所诱导变化之和的两倍。我们得出结论，这些PD变化主要是通过刺激空肠中的电致性Cl分泌而发生的。进一步利用这种方法来测定PD可能对肠道分泌的临床研究有用。
