Koike K, Ohnuki T, Ohkuda K, Nitta S, Nakada T
Tohoku J Exp Med. 1986 Jul;149(3):293-305. doi: 10.1620/tjem.149.293.
The purpose of this investigation is to analyze the branching pattern of pulmonary artery. The excised right lower lobes of six mongrel dogs were fixed by perfusion with 1.2% glutaraldehyde solution. Pressure of the pulmonary artery, vein and airway were controlled during perfusion. After fixation, resin was injected manually into the artery. When resin was completely polymerized, the lung was placed in 15 N NaOH solution to remove lung tissue. The diameters and lengths of branches were measured by stereomicroscopy. The results obtained were as follows: The basically dichotomous pulmonary arteries showed not only fairly regular dichotomies but monopodies, in the latter of which the diameter of parent branch was almost the same as that of its larger daughter branch. The diameter of monopodial branch was less than 500 microns, even when the parent branch was a large central segment. In arteries of over 1,000 microns in diameter, 75% or more of branchings were monopodies, whereas in small arteries of 200-300 microns in diameter, half the bifurcations were monopodies. At a bifurcation, the average daughter-to-parent diameter ratio was 0.8-0.9 for the larger daughter branch and 0.5 for the smaller one. The average length of branches was in proportion to their average diameter.
本研究的目的是分析肺动脉的分支模式。对6只杂种犬的右下叶进行切除，并用1.2%的戊二醛溶液灌注固定。灌注过程中控制肺动脉、肺静脉和气道的压力。固定后，手动将树脂注入动脉。当树脂完全聚合后，将肺置于15N的氢氧化钠溶液中以去除肺组织。通过体视显微镜测量分支的直径和长度。得到的结果如下：基本呈二叉状的肺动脉不仅有相当规则的二叉分支，还有单干分支，在单干分支中，母支的直径与其较大子支的直径几乎相同。即使母支是大的中央段，单干分支的直径也小于500微米。在直径超过1000微米的动脉中，75%或更多的分支是单干分支，而在直径为200 - 300微米的小动脉中，一半的分叉是单干分支。在分叉处，较大子支的平均子支与母支直径比为0.8 - 0.9，较小子支的平均子支与母支直径比为0.5。分支的平均长度与其平均直径成比例。
