Department of Hematology and Institute of Hematology, West China Hospital, Sichuan University, Chengdu, 610041, China.
Stem Cells and Regenerative Medicine Research Center, Sichuan Stem Cell Bank/Sichuan Neo-Life Stem Cell Biotech Inc., 15 Jinquan Road, Chengdu, 610036, China.
Stem Cell Res Ther. 2023 Aug 30;14(1):224. doi: 10.1186/s13287-023-03417-1.
Mesenchymal stem cells (MSCs) are a class of adult stem cells with self-renewal and multidirectional differentiation potential that may be a treatment for aplastic anemia (AA).
Umbilical cord-derived MSCs were cultured in three media (Mesencult-XF, MCL, and StemPro MSC SFM CTS). HGF, PGE2, ANG-1, TGF-β1, IFN-γ, and TNF-α were detected using ELISA. The AA mouse model was built via post-irradiation lymphocyte infusion. After different treatments, routine blood, VEGF, and Tregs were detected every week. On day 28, all mice were killed, and their femurs were stained with HE.
Umbilical cord-derived MSCs cultured in the three media all conformed to the general characteristics of MSCs. HGF secreted by MSCs in the Mesencult-XF, and MCL was greater than that in the StemPro MSC SFM CTS; ANG-1 and TGF-β1 in the MCL were more than that in Mesencult-XF and StemPro MSC SFM CTS; PGE2 in the MCL and StemPro MSC SFM CTS was more than that in the Mesencult-XF. MSCs in the MCL and StemPro MSC SFM CTS inhibited IFN-γ and TNF-α more than those in the Mesencult-XF. The peripheral blood cell in the AA groups was at a low level while that in the MSC group recovered rapidly. The Treg ratio and VEGF level in the MSC group were higher than those in the AA group. The bone marrow (BM) recovered significantly after MSC infusion.
MSCs in the MCL were advantageous in supporting hematopoiesis and modulating immunity and had the potential for effective treatment of AA.
间充质干细胞（MSCs）是一类具有自我更新和多向分化潜能的成体干细胞，可能是再生障碍性贫血（AA）的一种治疗方法。
从脐带中分离出的间充质干细胞在三种培养基（Mesencult-XF、MCL 和 StemPro MSC SFM CTS）中进行培养。使用 ELISA 检测 HGF、PGE2、ANG-1、TGF-β1、IFN-γ 和 TNF-α。通过辐照后淋巴细胞输注建立 AA 小鼠模型。在进行不同的治疗后，每周检测常规血液、VEGF 和 Tregs。第 28 天，处死所有小鼠，对其股骨进行 HE 染色。
在三种培养基中培养的脐带间充质干细胞均符合 MSCs 的一般特征。Mesencult-XF 和 MCL 中的 MSC 分泌的 HGF 大于 StemPro MSC SFM CTS；MCL 中的 ANG-1 和 TGF-β1 多于 Mesencult-XF 和 StemPro MSC SFM CTS；MCL 和 StemPro MSC SFM CTS 中的 PGE2 多于 Mesencult-XF。MCL 和 StemPro MSC SFM CTS 中的 MSC 抑制 IFN-γ 和 TNF-α的作用强于 Mesencult-XF 中的 MSC。AA 组的外周血细胞水平较低，而 MSC 组恢复迅速。MSC 组的 Treg 比例和 VEGF 水平高于 AA 组。MSC 输注后骨髓（BM）明显恢复。
MCL 中的 MSC 在支持造血和调节免疫方面具有优势，具有有效治疗 AA 的潜力。
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