培养基补充剂的比较确定了自我报告的无血清制剂中的血清成分。

Comparison of culture media supplements identifies serum components in self-reported serum-free preparations.

作者信息

Delabie Willem, Vandewalle Vicky, Seghers Sabrina, De Bleser Dominique, Vandekerckhove Philippe, Feys Hendrik B

机构信息

Transfusion Research Center, Belgian Red Cross Flanders, Ottergemsesteenweg 413, Ghent, 9000, Belgium.

Transfusion Innovation Center, Belgian Red Cross Flanders, Ottergemsesteenweg 413, Ghent, 9000, Belgium.

出版信息

Stem Cell Res Ther. 2025 Aug 26;16(1):454. doi: 10.1186/s13287-025-04561-6.

DOI:10.1186/s13287-025-04561-6

PMID:40859293

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12382287/

Abstract

BACKGROUND

Cell culture media are essential for cell expansion and many cells still depend on blood-derived supplements for optimal growth. From a regulatory perspective, these materials are ideally xeno-free, serum-free or even chemically defined. However, differences in composition and in performance are seldom clear from the terminology used in this field.

METHODS

Therefore, we set out to investigate the presence of serum in seven serum-free media (SFM) and link that information to the performance and cost. We used fetal bovine serum and five human platelet lysate preparations (hPL) for comparison.

RESULTS

Our data show significant differences in growth factor content between categories, but this did not correlate with mesenchymal stem cell (MSC) growth kinetics or maximal cell yield. Myeloperoxidase, glycocalicin and fibrinogen, derived from human leukocytes, platelets and plasma were detected at significant levels in 2 from 7 SFM. MSC cultured in these two SFM had a CD44-phenotype akin to hPL, essentially reclassifying them as hPL. Most SFM supported MSC expansion well, but some did not. In contract, all hPL supported MSC growth. The cost of SFM is significantly higher than hPL.

CONCLUSIONS

We conclude that terminology regarding serum presence can be misleading. The cost-performance balance is best for hPL at this moment.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1186/s13287-025-04561-6.

摘要

背景

细胞培养基对于细胞扩增至关重要，许多细胞仍依赖血液来源的补充剂以实现最佳生长。从监管角度来看，这些材料理想情况下应无动物源、无血清甚至是化学成分明确的。然而，从该领域使用的术语中，组成和性能的差异很少清晰明了。

方法

因此，我们着手研究七种无血清培养基（SFM）中血清的存在情况，并将该信息与性能和成本联系起来。我们使用胎牛血清和五种人血小板裂解物制剂（hPL）进行比较。

结果

我们的数据显示不同类别之间生长因子含量存在显著差异，但这与间充质干细胞（MSC）的生长动力学或最大细胞产量无关。在七种SFM中的两种中检测到了源自人白细胞、血小板和血浆的髓过氧化物酶、糖萼蛋白和纤维蛋白原，且含量显著。在这两种SFM中培养的MSC具有类似于hPL的CD44表型，基本上可将它们重新归类为hPL。大多数SFM能很好地支持MSC扩增，但有些则不能。相比之下，所有hPL都能支持MSC生长。SFM的成本明显高于hPL。