Sendelbach L E, Witschi H P, Tryka A F
Toxicol Appl Pharmacol. 1986 Sep 15;85(2):248-56. doi: 10.1016/0041-008x(86)90119-5.
The acute response in the lung of mice and rats exposed to an aerosol of BeSO4 was characterized by cell kinetics and histopathology. Animals were exposed for 1 hour in a nose-only chamber to a 13 microgram/liter chamber concentration of BeSO4 or to H2SO4 as control aerosol, and killed thereafter over a period of 21 days. Lung cell kinetics were evaluated as the labeling index (LI), defined as the percentage of cells labeled with tritiated thymidine. The labeled cells were also differentiated as to type. In rats, the LI showed a peak response on Day 8 following exposure, while the LI of mice showed a maximum response at Day 5. In rats, the proliferative response involved type II alveolar epithelial cells, interstitial and capillary endothelial cells. Histopathology showed type II alveolar cell hyperplasia and vacuolization of their cytoplasm, a thickened interstitium with infiltrates of interstitial macrophages and segmental leukocytes; a prominent feature was alveolar macrophages with ragged membranes. Three weeks after exposure, the interstitial response was largely resolved. In mice, the proliferative response was mainly found in the alveolar macrophage population and the interstitial and endothelial cells. Histopathological changes were similar to those found in rats, although less severe.
通过细胞动力学和组织病理学对暴露于硫酸铍气雾剂的小鼠和大鼠肺部的急性反应进行了表征。动物在仅鼻吸入式舱室中暴露1小时，暴露于浓度为13微克/升的硫酸铍舱室环境或作为对照气雾剂的硫酸中，此后在21天的时间段内处死。肺细胞动力学通过标记指数（LI）进行评估，标记指数定义为用氚标记的胸腺嘧啶核苷标记的细胞百分比。标记的细胞也按类型进行区分。在大鼠中，标记指数在暴露后第8天显示出峰值反应，而小鼠的标记指数在第5天显示出最大反应。在大鼠中，增殖反应涉及II型肺泡上皮细胞、间质和毛细血管内皮细胞。组织病理学显示II型肺泡细胞增生及其细胞质空泡化，间质增厚，伴有间质巨噬细胞和节段性白细胞浸润；一个突出特征是肺泡巨噬细胞膜粗糙。暴露三周后，间质反应基本消退。在小鼠中，增殖反应主要见于肺泡巨噬细胞群体以及间质和内皮细胞。组织病理学变化与大鼠相似，尽管程度较轻。
