测定肝癌细胞中肝癌衍生生长因子的可能靶基因。

Determination of the Possible Target Genes of Hepatoma-derived Growth Factor in Hepatoma Cells.

机构信息

Division of Hepatobiliary and Pancreatic Diseases, Department of Gastroenterology, Hyogo Medical University, Nishinomiya, Japan

Division of Hepatobiliary and Pancreatic Diseases, Department of Gastroenterology, Hyogo Medical University, Nishinomiya, Japan.

出版信息

In Vivo. 2023 Sep-Oct;37(5):1975-1979. doi: 10.21873/invivo.13294.

DOI:10.21873/invivo.13294

PMID:37652516

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10500524/

Abstract

BACKGROUND/AIM: We identified a new growth factor, hepatoma-derived growth factor (HDGF), which is a presumed growth-stimulating factor of hepatocellular carcinoma (HCC). Recently, we identified two microRNAs (miR-6072 and miR-3137) induced by HDGF, which were also found to be associated with the prognosis of HCC patients. This study aimed to identify the target genes of these HDGF-related microRNAs.

MATERIALS AND METHODS

A public database was searched for candidate target genes of HDGF-related microRNAs. Using the microarray system, the genes whose expression changed in response to HDGF administration were determined. Finally, a public cancer genomics database was searched for genes that were induced by HDGF and associated with the prognosis of HCC.

RESULTS

A total of 1,132 genes were identified as common target genes of the 2 HDGF-related microRNAs. Among these genes, a microarray system showed that the expression of 6 genes was increased (≥1.5-fold) or decreased (≤0.67-fold) after HDGF administration. Using a cancer genomics database, two of the six genes were found to be related to the prognosis of HCC. A high expression of alkylglycerone phosphate synthase (AGPS) was significantly associated with a poor survival (p=0.0025, 0.0063 and 0.0081 for the 1-, 3- and 5-year survival, respectively). A high expression of the shroom family member 4 (SHROOM4) gene was found to be significantly associated with a better survival (p=0.003, 0.0006 and 0.0006 for the 1-, 3- and 5-year survival, respectively).

CONCLUSION

This study identified potential target genes of HDGF-related microRNAs that were associated with the prognosis of HCC.

摘要

背景/目的：我们鉴定了一种新的生长因子，即肝癌衍生生长因子（HDGF），它被认为是肝癌（HCC）的生长刺激因子。最近，我们鉴定了两种由 HDGF 诱导的 microRNAs（miR-6072 和 miR-3137），它们也与 HCC 患者的预后相关。本研究旨在鉴定这些与 HDGF 相关的 microRNAs 的靶基因。

材料和方法

在公共数据库中搜索与 HDGF 相关 microRNAs 的候选靶基因。使用 microarray 系统，确定了对 HDGF 给药有反应的基因表达变化。最后，在公共癌症基因组学数据库中搜索了由 HDGF 诱导且与 HCC 预后相关的基因。

结果

共鉴定出 1132 个基因是这两种与 HDGF 相关的 microRNAs 的共同靶基因。在这些基因中，microarray 系统显示，有 6 个基因的表达在 HDGF 给药后增加（≥1.5 倍）或减少（≤0.67 倍）。使用癌症基因组学数据库，发现这 6 个基因中的两个与 HCC 的预后相关。烷基甘油磷酸合酶（AGPS）的高表达与生存不良显著相关（p=0.0025、0.0063 和 0.0081 分别为 1、3 和 5 年生存率）。shroom 家族成员 4（SHROOM4）基因的高表达与生存改善显著相关（p=0.003、0.0006 和 0.0006 分别为 1、3 和 5 年生存率）。