Centre for Computational Biology & Bioinformatics, Central University of Himachal Pradesh, Kangra 176206, Himachal Pradesh, India.
Department of Environmental Studies, University of Delhi 110007, Delhi, India.
Pathog Dis. 2023 Jan 17;81. doi: 10.1093/femspd/ftad020.
Metalloproteins and metal-based inhibitors have been shown to effectively combat infectious diseases, particularly those caused by RNA viruses. In this study, a diverse set of bioinformatics methods was employed to identify metal-binding proteins of human RNA viruses. Seventy-three viral proteins with a high probability of being metal-binding proteins were identified. These proteins included 40 zinc-, 47 magnesium- and 14 manganese-binding proteins belonging to 29 viral species and eight significant viral families, including Coronaviridae, Flaviviridae and Retroviridae. Further functional characterization has revealed that these proteins play a critical role in several viral processes, including viral replication, fusion and host viral entry. They fall under the essential categories of viral proteins, including polymerase and protease enzymes. Magnesium ion is abundantly predicted to interact with these viral enzymes, followed by zinc. In addition, this study also examined the evolutionary aspects of predicted viral metalloproteins, offering essential insights into the metal utilization patterns among different viral species. The analysis indicates that the metal utilization patterns are conserved within the functional classes of the proteins. In conclusion, the findings of this study provide significant knowledge on viral metalloproteins that can serve as a valuable foundation for future research in this area.
金属蛋白和基于金属的抑制剂已被证明可有效对抗传染病，特别是由 RNA 病毒引起的传染病。在这项研究中，采用了多种生物信息学方法来鉴定人类 RNA 病毒的金属结合蛋白。确定了 73 种极有可能是金属结合蛋白的病毒蛋白。这些蛋白包括属于 29 种病毒物种和 8 种重要病毒科的 40 种锌结合蛋白、47 种镁结合蛋白和 14 种锰结合蛋白，其中包括冠状病毒科、黄病毒科和逆转录病毒科。进一步的功能特征分析表明，这些蛋白在几种病毒过程中发挥关键作用，包括病毒复制、融合和宿主病毒进入。它们属于病毒蛋白的必需类别，包括聚合酶和蛋白酶酶。大量预测镁离子与这些病毒酶相互作用，其次是锌。此外，这项研究还研究了预测的病毒金属蛋白的进化方面，为不同病毒物种之间的金属利用模式提供了重要的见解。分析表明，金属利用模式在蛋白的功能类别内是保守的。总之，这项研究的结果提供了关于病毒金属蛋白的重要知识，可为该领域的未来研究提供有价值的基础。
