Weeks B A, Warinner J E
Vet Immunol Immunopathol. 1986 Jun;12(1-4):313-20. doi: 10.1016/0165-2427(86)90135-2.
The chemotactic and phagocytic efficiencies of macrophages from spot, Leiostomus xanthurus and hogchoker, Trinectes maculatus are reported here. Fish were captured from the York and Ware Rivers, two relatively nonpolluted rivers, and from a reach of the Elizabeth River, known to be contaminated with high concentrations of polynuclear aromatic hydrocarbons (PAHs). Kidney cell types were separated by density gradient centrifugation and single cell suspensions of macrophages were used for in vitro studies. Chemotactic activity was measured in Boyden chambers using Escherichia coli as the chemotactic stimulus. The percentage of macrophages migrating was maximal at 90 min but was markedly reduced in Elizabeth River fish (spot, 33%; hogchoker, 56%) as compared to clean water controls (spot, 55%; hogchoker, 85%). Macrophage phagocytosis of bacteria was quantified by the microscopic enumeration of the proportion of phagocytes which contained intracellular bacteria. Phagocytic activity reached maximum values within 120 min and remained relatively constant for the remainder of the experiment. Phagocytic activity was reduced significantly in the fish obtained from the Elizabeth River. Values at 120 min were 74% and 19% for spot from the control and PAH-contaminated sites, respectively. Comparable values for hogchoker were 88% and 32%. The results suggest that defective macrophage function is related to exposure to PAH-contaminated sediments.
本文报道了斑点鳎（Leiostomus xanthurus）和斑鳍沙塘鳢（Trinectes maculatus）巨噬细胞的趋化和吞噬效率。鱼取自约克河和韦尔河这两条相对未受污染的河流，以及伊丽莎白河的一段已知受高浓度多环芳烃（PAHs）污染的河段。通过密度梯度离心分离肾细胞类型，并将巨噬细胞单细胞悬液用于体外研究。使用大肠杆菌作为趋化刺激物，在博伊登小室中测量趋化活性。巨噬细胞迁移的百分比在90分钟时达到最大值，但与清洁水对照组相比，伊丽莎白河鱼类（斑点鳎为33%；斑鳍沙塘鳢为56%）显著降低（斑点鳎清洁水对照组为55%；斑鳍沙塘鳢清洁水对照组为85%）。通过显微镜计数含有细胞内细菌的吞噬细胞比例来量化巨噬细胞对细菌的吞噬作用。吞噬活性在120分钟内达到最大值，并在实验剩余时间内保持相对稳定。从伊丽莎白河获得的鱼的吞噬活性显著降低。在120分钟时，来自对照位点和PAH污染位点的斑点鳎的值分别为74%和19%。斑鳍沙塘鳢的可比数值为88%和32%。结果表明，巨噬细胞功能缺陷与暴露于PAH污染的沉积物有关。
