Romero Paco, Lafuente María Teresa
Department of Food Biotechnology, Institute of Agrochemistry and Food Technology (IATA-CSIC), Avenida Catedrático Agustín Escardino 7, 46980 Paterna, Valencia, Spain.
Plants (Basel). 2023 May 22;12(10):2062. doi: 10.3390/plants12102062.
Fruit nutritional value, plant growth, and yield can be compromised by deficient copper (Cu) bioavailability, which often appears in arable lands. This condition causes low Cu content and modifications in the ripening-associated processes in tomato fruit. This research studies the transcriptomic changes that occur in red ripe tomato fruit grown under suboptimal Cu conditions to shed light on the molecular mechanisms underlying this stress. Comparative RNA-sequencing and functional analyses revealed that Cu deficiency during cultivation activates signals for metal ion transport, cellular redox homeostasis, pyridoxal phosphate binding, and amino acid metabolism while repressing the response to phosphate starvation in harvested fruit. Transcriptomic analyses highlighted a number of novel Cu stress-responsive genes of unknown function and indicated that Cu homeostasis regulation in tomato fruit may involve additional components than those described in model plants. It also studied the regulation of high-affinity Cu transporters and a number of well-known Cu stress-responsive genes during tomato fruit ripening depending on Cu availability, which allowed potential candidates to be targeted for biotechnological improvements in reproductive tissues. We provide the first study characterizing the molecular responses of fruit to Cu deficiency stress for any fruit crop.
水果的营养价值、植物生长和产量会因铜（Cu）生物有效性不足而受到影响，这种情况在耕地上经常出现。这种状况会导致番茄果实中的铜含量降低，并使与成熟相关的过程发生改变。本研究探究了在铜条件欠佳的情况下生长的红色成熟番茄果实中发生的转录组变化，以揭示这种胁迫背后的分子机制。比较RNA测序和功能分析表明，栽培过程中的铜缺乏会激活金属离子转运、细胞氧化还原稳态、磷酸吡哆醛结合和氨基酸代谢的信号，同时抑制收获果实中对磷饥饿的反应。转录组分析突出了一些功能未知的新型铜胁迫响应基因，并表明番茄果实中的铜稳态调节可能涉及比模式植物中描述的更多的成分。该研究还探讨了在番茄果实成熟过程中，根据铜的可利用性对高亲和力铜转运蛋白和一些著名的铜胁迫响应基因的调控，这使得潜在的候选基因可被用于生殖组织的生物技术改良。我们首次对任何水果作物果实对铜缺乏胁迫的分子反应进行了表征研究。
