通过竞争性半胱氨酸组分析发现顺铂结合蛋白

Discovery of cisplatin-binding proteins by competitive cysteinome profiling.

作者信息

Wang Xianghe, Zhang Yihai, Wang Chu

机构信息

Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University Beijing China

Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University Beijing China.

出版信息

RSC Chem Biol. 2023 Aug 1;4(9):670-674. doi: 10.1039/d3cb00042g. eCollection 2023 Aug 30.

DOI:10.1039/d3cb00042g

PMID:37654507

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10467758/

Abstract

Cisplatin is a widely used cancer metallodrug that induces cytotoxicity by targeting DNA and chelating cysteines in proteins. Here we applied a competitive activity-based protein profiling strategy to identify cisplatin-binding cysteines in cancer proteomes. A novel cisplatin target, MetAP1, was identified and functionally validated to contribute to cisplatin's cytotoxicity.

摘要

顺铂是一种广泛应用的癌症金属药物，它通过靶向DNA和螯合蛋白质中的半胱氨酸来诱导细胞毒性。在此，我们应用了一种基于竞争活性的蛋白质谱分析策略，以鉴定癌症蛋白质组中与顺铂结合的半胱氨酸。我们鉴定出了一种新的顺铂靶点——甲硫氨酸氨肽酶1（MetAP1），并对其功能进行了验证，结果表明它有助于顺铂的细胞毒性作用。