脉冲采样荧光寿命成像：光子经济性评估
Pulse-sampling fluorescence lifetime imaging: evaluation of photon economy.
作者信息
Zhou Xiangnan, Bec Julien, Ehrlich Katjana, Garcia Alba Alfonso, Marcu Laura
出版信息
Opt Lett. 2023 Sep 1;48(17):4578-4581. doi: 10.1364/OL.490096.
Abstract
This Letter presents an experimental study comparing the photon rate and photon economy of pulse sampling fluorescence lifetime imaging (PS-FLIm) with the conventional time-correlated single photon counting (TCSPC) technique. We found that PS-FLIm has a significantly higher photon detection rate (200 MHz) compared with TCSPC (2-8 MHz) but lower photon economy (4-5 versus 1-1.3). The main factor contributing to the lower photon economy in PS-FLIm is laser pulse variability. These results demonstrate that PS-FLIm offers 25× faster imaging speed than TCSPC while maintaining room light rejection in clinical settings. This makes PS-FLIm a robust technique for clinical applications.
摘要
本信函展示了一项实验研究，该研究比较了脉冲采样荧光寿命成像（PS-FLIm）与传统时间相关单光子计数（TCSPC）技术的光子速率和光子经济性。我们发现，与TCSPC（2-8MHz）相比，PS-FLIm具有显著更高的光子检测率（200MHz），但光子经济性较低（4-5对1-1.3）。导致PS-FLIm中光子经济性较低的主要因素是激光脉冲的可变性。这些结果表明，PS-FLIm在临床环境中保持对室内光线的抑制能力的同时，成像速度比TCSPC快25倍。这使得PS-FLIm成为一种适用于临床应用的强大技术。
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