Liu Xuhui, Bec Julien, Zhou Xiangnan, Garcia Alba Alfonso, Marcu Laura
Opt Lett. 2025 Feb 1;50(3):900-903. doi: 10.1364/OL.547582.
We report a multispectral laser-scanning pulse-sampling fluorescence lifetime imaging (LSPS-FLIm) system designed for rapid, high-resolution imaging of large tissue specimens. This system provides a substantial imaging field of view (FoV) of 6 × 15 cm with a high spatial resolution of ∼17.5 µm. The LSPS-FLIm system has been tested on a range of fluorescent dyes, endogenous tissue fluorophores, and tissue specimens with varied sizes and properties. These tests demonstrate the system's versatility in resolving morphological and molecular features, enabling centimeter-scale FoV imaging, and distinguishing complex microstructures in tissue specimens. With the capability to maintain high imaging quality and acquisition speed while minimizing tissue damage, LSPS-FLIm represents a promising advancement in the field of fluorescence lifetime imaging for biological and clinical applications.
我们报告了一种多光谱激光扫描脉冲采样荧光寿命成像（LSPS-FLIm）系统，该系统专为大型组织标本的快速、高分辨率成像而设计。该系统提供了6×15厘米的大成像视野（FoV），具有约17.5微米的高空间分辨率。LSPS-FLIm系统已在一系列荧光染料、内源性组织荧光团以及具有不同大小和特性的组织标本上进行了测试。这些测试证明了该系统在解析形态和分子特征、实现厘米级视野成像以及区分组织标本中的复杂微观结构方面的多功能性。LSPS-FLIm能够在尽量减少组织损伤的同时保持高成像质量和采集速度，代表了荧光寿命成像领域在生物和临床应用方面的一项有前景的进展。
