Jiangsu Ocean University Jiangsu Key Laboratory of Marine Bioresources and Environment /Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province /School of Marine Science and Fisheries, Lianyungang 222005, Jiangsu, China.
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, Jiangsu, China.
Lett Appl Microbiol. 2023 Sep 1;76(9). doi: 10.1093/lambio/ovad098.
Fusarium head blight (FHB) is a wheat disease caused by the plant pathogen Fusarium graminearum, which leads to crop yield losses and agricultural economic losses, as well as poses a threat to the environment and human health. Effective biocontrol of F. graminearum is urgent. An antagonistic strain HZ-5 with 59.2% antagonistic activity against F. graminearum in vitro had been isolated from sea mud of Haizhou Bay using a dual-culture assay, which was highly homologous with Bacillus halosaccharovorans according to the 16S rRNA sequence. The antagonistic activity of HZ-5 had been further studied. HZ-5 had a broad range of antagonistic activity against another six plant pathogenic fungi and was effective in controlling FHB of wheat in pot experiment. The substances with antagonistic activity were temperature insensitive, and had been purified by HPLC (High Performance Liquid Chromatography) to prove to be secreted lipopeptides. The antagonistic substances induced the biosynthesis of chitin and glycerol, while ergosterol , cholesterol, and phosphatidylcholine reduced their inhibitory effects on F. graminearum. These data would be helpful to provide a better biocontrol strain against FHB, and to provide important basis to elucidate the antagonistic mechanism of biocontrol.
镰刀菌穗腐病（FHB）是一种由植物病原菌禾谷镰刀菌引起的小麦病害，导致作物产量损失和农业经济损失，同时对环境和人类健康构成威胁。有效控制禾谷镰刀菌是当务之急。采用双培养法从海州湾海泥中分离出一株对禾谷镰刀菌具有 59.2%体外拮抗活性的拮抗菌株 HZ-5，根据 16S rRNA 序列，该菌株与芽孢杆菌属 halo saccharovorans 高度同源。进一步研究了 HZ-5 的拮抗活性。HZ-5 对另外六种植物病原真菌具有广泛的拮抗活性，在盆栽试验中对小麦赤霉病有防治效果。具有拮抗活性的物质对温度不敏感，并已通过高效液相色谱（HPLC）进行了纯化，证明是分泌的脂肽。拮抗物质诱导几丁质和甘油的生物合成，而麦角固醇、胆固醇和磷脂酰胆碱降低了它们对禾谷镰刀菌的抑制作用。这些数据将有助于提供更好的防治 FHB 的生物控制菌株，并为阐明生物防治的拮抗机制提供重要依据。
