Microbiology unit, Foch Hospital, Suresnes, France.
Microbiology unit, Foch Hospital, Suresnes, France.
Diagn Microbiol Infect Dis. 2023 Nov;107(3):116044. doi: 10.1016/j.diagmicrobio.2023.116044. Epub 2023 Aug 2.
Identification of mycobacteria is crucial for clinical management of patients. The new MBT Mycobacteria kit from the Easy MycoEx protocol (Bruker) is used for the identification of mycobacteria by MALDI Biotyper. The Easy MycoEx protocol was compared to the MycoEx protocol (1) for identification of various mycobacterial isolates collected from samples in 2021, (2) for prospective identification on primary culture during 2 periods. For 44 isolates in MGIT broth, identification rates were high and similar for both protocols (98% vs 95% at cut-off 1.6 and 91% vs 82% at cut-off 1.8). For 42 mycobacteria on Coletsos agar, identification rates were 88% versus 90% at cut-off 1.6 and 76% for both protocols at cut-off 1.8. For slow-growing mycobacteria in MGIT, reproducibility of deposit results was superior with Easy MycoEx. No difference of score was observed between 2 protocols performed on primary culture. Clinical laboratories can easily implement the Easy MycoEx protocol.
鉴定分枝杆菌对于患者的临床管理至关重要。来自 Bruker 的 Easy MycoEx 方案的新型 MBT 分枝杆菌试剂盒用于通过 MALDI Biotyper 鉴定分枝杆菌。比较了 Easy MycoEx 方案与 MycoEx 方案：（1）用于鉴定 2021 年从样本中收集的各种分枝杆菌分离株，（2）用于 2 个时期的初代培养物的前瞻性鉴定。对于 MGIT 肉汤中的 44 株分离株，两种方案的鉴定率均较高且相似（截止值为 1.6 时为 98%比 95%，截止值为 1.8 时为 91%比 82%）。对于 Coletsos 琼脂上的 42 株分枝杆菌，截止值为 1.6 时的鉴定率分别为 88%和 90%，截止值为 1.8 时两种方案的鉴定率均为 76%。对于 MGIT 中的生长缓慢的分枝杆菌，Easy MycoEx 的沉积结果重现性更好。两种方案在初代培养物上的评分无差异。临床实验室可以轻松实施 Easy MycoEx 方案。
