Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou, Guangdong 510640, China.
Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou, Guangdong 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, Guangdong, China.
Food Chem. 2024 Jan 30;432:137272. doi: 10.1016/j.foodchem.2023.137272. Epub 2023 Aug 25.
Pesticide residues have raised considerable concern about environmental health and food safety. Despite a great advance in enzymatic sensors for pesticide detection, the intrinsic fragility of native enzyme and possible fake results due to single mode signal have hindered its wide application. Here, a novel dual-mode sensor is reported for organophosphorus pesticide detection by using metal-organic framework (MOF) nanozyme NH-CuBDC as sensing element. The intrinsic peroxidase-mimicking activity and fluorescence property of NH-CuBDC enable both colorimetric and fluorescent detection of chlorpyrifos. Compared with previously reported chlorpyrifos sensors, our sensor exhibits outstanding sensitivity, and the limits of detection (LOD, S/N = 3) in colorimetric and fluorescent modes are 1.57 ng/mL and 2.33 ng/mL, respectively. No obvious interferences from other substances were measured and chlorpyrifos analysis in real samples presented good reliability, showing practical potential. This work is anticipated to provide new insights to develop multifunctional nanozymes and integrated multi-mode sensing platforms.
农药残留引起了人们对环境健康和食品安全的极大关注。尽管在用于农药检测的酶传感器方面取得了重大进展，但天然酶的固有脆弱性以及单一模式信号可能导致的虚假结果，阻碍了其广泛应用。本研究报道了一种新型的双模式传感器，用于有机磷农药的检测，该传感器使用金属有机骨架（MOF）纳米酶 NH-CuBDC 作为传感元件。NH-CuBDC 具有内在的过氧化物酶模拟活性和荧光特性，可用于对毒死蜱进行比色和荧光检测。与之前报道的毒死蜱传感器相比，我们的传感器表现出优异的灵敏度，在比色和荧光模式下的检测限（LOD，S/N=3）分别为 1.57ng/mL 和 2.33ng/mL。未检测到其他物质的明显干扰，并且对实际样品中的毒死蜱进行分析显示出良好的可靠性，具有实际应用潜力。这项工作有望为开发多功能纳米酶和集成多模式传感平台提供新的思路。
