狂犬病病毒基质蛋白mRNA的克隆及其氨基酸序列的测定。

Cloning of rabies virus matrix protein mRNA and determination of its amino acid sequence.

作者信息

Rayssiguier C, Cioe L, Withers E, Wunner W H, Curtis P J

出版信息

Virus Res. 1986 Aug;5(2-3):177-90. doi: 10.1016/0168-1702(86)90016-x.

Abstract

A cDNA clone of mRNA for rabies virus matrix (M) protein has been identified. The clone hybridizes to an mRNA species from rabies virus-infected cells, whose size correlates to the size of the M protein in rabies virions, and selects an mRNA that translates into a polypeptide corresponding in size to M protein. The nucleotide sequence of the cloned cDNA was determined and from this a complete amino acid sequence for M protein was deduced. The deduced sequence of 202 amino acids bears no detectable sequence homology with vesicular stomatitis virus M protein although these proteins may share functional homology.

摘要

已鉴定出狂犬病毒基质（M）蛋白mRNA的一个cDNA克隆。该克隆与来自狂犬病毒感染细胞的一种mRNA杂交，其大小与狂犬病毒粒子中M蛋白的大小相关，并选择出一种能翻译成与M蛋白大小相对应的多肽的mRNA。测定了克隆cDNA的核苷酸序列，并由此推导了M蛋白的完整氨基酸序列。推导的202个氨基酸的序列与水疱性口炎病毒M蛋白没有可检测到的序列同源性，尽管这些蛋白可能具有功能同源性。