Sullivan D C, Atherton S S, Caughman G B, Staczek J, O'Callaghan D J
Virus Res. 1986 Aug;5(2-3):201-12. doi: 10.1016/0168-1702(86)90018-3.
Infection of nonpermissive primary hamster embryo cells with equine herpesvirus type 3 (EHV-3; multiplicity of infection = 10 pfu/cell) resulted in an abortive infection and the development of several hundred foci of rapidly growing cells. Five of these foci were chosen at random for the establishment of transformed cell lines, designated EVD-1 (equine venereal disease) through 5. These transformed cell lines exhibited altered biological properties typical of transformed cells, including immortality, growth to high saturation density, colony formation in soft agar, reduced serum requirements, aneuploid karyotype, and oncogenicity in syngeneic animals. Subsequently, five corresponding tumor cell lines (EVD-1T through 5T) with similar biological properties were established. All EHV-3 transformed and tumor cell lines have been shown to express EHV-3-specific proteins by indirect immunofluorescence assays employing rabbit antisera to EHV-3 infected equine cells. None of the transformed cell lines were found to release infectious virus by infectious center or cocultivation assay or to contain viral particles by electron microscopy.
用3型马疱疹病毒（EHV - 3；感染复数=10个蚀斑形成单位/细胞）感染非允许性原代仓鼠胚胎细胞，导致了一次流产感染以及数百个快速生长细胞灶的形成。随机选择其中5个细胞灶用于建立转化细胞系，命名为EVD - 1至5（马性病）。这些转化细胞系表现出典型的转化细胞改变后的生物学特性，包括永生性、生长至高饱和密度、在软琼脂中形成集落、降低血清需求、非整倍体核型以及在同基因动物中的致瘤性。随后，建立了具有相似生物学特性的5个相应肿瘤细胞系（EVD - 1T至5T）。通过使用针对感染EHV - 3的马细胞的兔抗血清进行间接免疫荧光测定，已证明所有EHV - 3转化细胞系和肿瘤细胞系均表达EHV - 3特异性蛋白。通过感染中心或共培养测定未发现任何转化细胞系释放传染性病毒，并且通过电子显微镜也未发现其含有病毒颗粒。
