Katoh I, Yoshinaka Y, Luftig R B
Virus Res. 1986 Aug;5(2-3):265-76. doi: 10.1016/0168-1702(86)90023-7.
Cerulenin is an antibiotic that interferes with fatty acid synthesis in eukaryotic cells. It had been shown by Schultz and Oroszlan (1983), that murine leukemia virus (MuLV) Pr65gag, the polyprotein precursor to the virion core proteins contains the fatty acid myristate at its NH2 terminus. We showed that when 20 micrograms/ml of cerulenin is added for 3 h to mouse fibroblasts chronically infected with Moloney (M)-MuLV it causes a greater than 4-fold decrease in virus production. This is accompanied by an accumulation of uncleaved Pr65gag in the infected cells. Further, thin-section electron micrographs of cerulenin-treated cells show a 2-fold increase in the number of nascent-budding forms, as well as the appearance of aberrant viral forms at the cell membrane. This suggests that the failure to add myristic acid to Pr65gag prevents their proper assembly into viral particles.
浅蓝菌素是一种干扰真核细胞脂肪酸合成的抗生素。舒尔茨和奥罗斯兰（1983年）已证明，鼠白血病病毒（MuLV）的Pr65gag，即病毒体核心蛋白的多蛋白前体，在其NH2末端含有脂肪酸肉豆蔻酸。我们发现，当向长期感染莫洛尼（M）-MuLV的小鼠成纤维细胞中添加20微克/毫升浅蓝菌素3小时时，病毒产量会下降超过4倍。这伴随着未切割的Pr65gag在感染细胞中的积累。此外，经浅蓝菌素处理的细胞的超薄切片电子显微镜照片显示，新生出芽形式的数量增加了2倍，同时细胞膜上出现了异常病毒形式。这表明未能将肉豆蔻酸添加到Pr65gag中会阻止它们正确组装成病毒颗粒。
