Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Sci Total Environ. 2023 Dec 15;904:166759. doi: 10.1016/j.scitotenv.2023.166759. Epub 2023 Aug 31.
A bacterial consortium, termed WPB, was obtained from polycyclic aromatic hydrocarbons (PAHs) contaminated soil from a coking site. The consortium effectively degraded 100 mg L pyrene by 94.8 % within 12 days. WPB was also able to degrade phenanthrene (98.3 %) and benzo[a]pyrene (24.6 %) in 12 days, while the individual isolates showed no PAHs degrading ability. Paracoccus sp. dominated the bacterial consortium (65.0-86.2 %) throughout the degradation process. Metagenomic sequencing reveals the proportion of sequences with xenobiotics biodegradation and metabolism increased throughout the degradation process indicating the great potential of WPB to degrade pollutants. The annotation of genes by metagenomic analysis help reconstruct the degradation pathways ("phthalate pathway" and "naphthalene degradation") and reveal how different bacteria contribute to the degradation process. Mycobacterium gilvum was found to carry nidAB genes that catalyze the first step of high-molecular-weight (HMW) PAHs in the degradation process despite Mycobacterium gilvum accounting for only 0.005-0.06 %. In addition, genomes of Paracoccus denitrificans and some other genera affiliated with Devosia, Pusillimonas caeni and Eoetvoesia caeni were successfully recovered and were found to carry genes responsible for the degradation of the intermediates of pyrene. These results enable further understanding of the metabolic patterns of pyrene-degrading consortia and provide direction for further cultivation and discovery of key players in complex microbial consortia.
从焦化场地多环芳烃（PAHs）污染土壤中获得了一个名为 WPB 的细菌共生体。该共生体在 12 天内有效地降解了 100mg/L 的芘，降解率达到 94.8%。WPB 还能够在 12 天内降解菲（98.3%）和苯并[a]芘（24.6%），而单个分离株则没有 PAHs 降解能力。假单胞菌属在整个降解过程中占细菌共生体的 65.0-86.2%。宏基因组测序显示，随着降解过程的进行，具有外来生物生物降解和代谢能力的序列比例增加，表明 WPB 具有很大的降解污染物的潜力。通过宏基因组分析对基因进行注释有助于重建降解途径（“邻苯二甲酸酯途径”和“萘降解途径”），并揭示不同细菌如何参与降解过程。尽管吉尔姆分枝杆菌仅占 0.005-0.06%，但发现吉尔姆分枝杆菌携带 nidAB 基因，该基因可催化降解过程中高分子量（HMW）PAHs 的第一步。此外，成功回收了 Paracoccus denitrificans 和一些其他属的基因组，这些属与 Devosia、Pusillimonas caeni 和 Eoetvoesia caeni 有关，并发现它们携带负责降解芘中间产物的基因。这些结果使我们能够进一步了解芘降解共生体的代谢模式，并为进一步培养和发现复杂微生物共生体中的关键成员提供了方向。
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