Wu Xing, Tao Yiqing, Ren Yangguang, Zhang Zihao, Zhao Yudian, Tian Yixiang, Li Yijie, Hou Meng, Guo Yulong, Gong Yujie, Zhang Yanhua, Li Donghua, Li Hong, Jiang Ruirui, Li Guoxi, Liu Xiaojun, Kang Xiangtao, Tian Yadong
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China.
Poult Sci. 2023 Nov;102(11):103028. doi: 10.1016/j.psj.2023.103028. Epub 2023 Aug 16.
It has been reported that adiponectin (AdipoQ), an adipokine secreted by white adipose tissue, plays an important role in the control of animal reproduction in addition to its function in energy homeostasis by binding to its receptors AdipoR1/2. However, the molecular mechanisms of AdipoQ in the regulation of animal reproduction remain elusive. In this study, we investigated the effects of AdipoQ on hypothalamic reproductive hormone (GnRH) secretion and reproduction-related receptor gene (estrogen receptor [ER] and progesterone receptor [PR]) expression in hypothalamic neuronal cells (HNCs) of chickens by using real-time fluorescent quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), Western blot (WB) and cell counting kit-8 (CCK-8) assays and found that overexpression of AdipoQ could increase the expression levels of AdipoR1/2 and reproduction-related receptor genes (P < 0.05) while decreasing the expression level of GnRH. In contrast, interference with AdipoQ mRNA showed the opposite results in HNCs. Furthermore, we demonstrated that AdipoQ exerts its functions through the AMPK and PI3K signaling pathways. Finally, our in vitro experiments found that AdipoRon (a synthetic substitute for AdipoQ) treatment and AdipoR1/2 RNAi interference co-treatment resulted in no effect on GnRH secretion, suggesting that the inhibition of GnRH secretion by AdipoQ is mediated by the AdipoR1/2 signaling axis. In summary, we uncovered, for the first time, the molecular mechanism of AdipoQ in the regulation of reproductive hormone secretion in hypothalamic neurons in chickens.
据报道，脂联素（AdipoQ）是一种由白色脂肪组织分泌的脂肪因子，除了通过与受体AdipoR1/2结合在能量稳态中发挥作用外，在动物繁殖控制中也起着重要作用。然而，AdipoQ在动物繁殖调节中的分子机制仍不清楚。在本研究中，我们通过实时荧光定量PCR（RT-qPCR）、酶联免疫吸附测定（ELISA）、蛋白质免疫印迹（WB）和细胞计数试剂盒-8（CCK-8）试验，研究了AdipoQ对鸡下丘脑神经元细胞（HNCs）中下丘脑生殖激素（GnRH）分泌以及生殖相关受体基因（雌激素受体[ER]和孕激素受体[PR]）表达的影响，发现AdipoQ的过表达可增加AdipoR1/2和生殖相关受体基因的表达水平（P<0.05），同时降低GnRH的表达水平。相反，干扰AdipoQ mRNA在HNCs中显示出相反的结果。此外，我们证明AdipoQ通过AMPK和PI3K信号通路发挥其功能。最后，我们的体外实验发现，AdipoRon（AdipoQ的合成替代物）处理和AdipoR1/2 RNAi干扰共同处理对GnRH分泌没有影响，这表明AdipoQ对GnRH分泌的抑制作用是由AdipoR1/2信号轴介导的。总之，我们首次揭示了AdipoQ在调节鸡下丘脑神经元生殖激素分泌中的分子机制。
