Su Yong, Huang Jiabin, Guo Qiaosheng, Shi Hongzhuan, Wei Min, Wang Chengxiang, Zhao Kun, Bao Tao
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China.
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China.
Gene. 2023 Dec 20;888:147752. doi: 10.1016/j.gene.2023.147752. Epub 2023 Sep 1.
Isatis indigotica Fortune is a plant species containing lignan compounds of significant economic value. Its root plays a crucial role in treating viruses and exhibits antitumor, anti-inflammatory, antibacterial, and other biological activities. Now, I. indigotica has been included in Isatis tinctoria Linnaeus. In this study, the roots of diploid I. indigotica, tetraploid I. indigotica, and Isatis tinctoria Linnaeus were analyzed using metabolome and transcriptome analysis. The metabolomic analysis detected 48 lignan metabolites, including Lirioresinol A, Vladinol A, Syringaresinol, Arctigenin, Acanthoside B, and Sesamin as characteristic compounds, without significant variations among the remaining metabolites. The transcriptomic analysis identified 41 differentially expressed phenylpropanoid synthase genes, which were further analyzed for variations in lignan transcriptome profiles across different samples. RT-qPCR analysis also revealed differential genes expression related to lignan biosynthesis pathway among the three sample groups. The analysis of transcription factors showed that the AP2-EREBP family (Iin24319), MYB family (Iin24843), and WRKY family (Iin08158) displayed expression patterns similar to Iin14549. Phylogenetic analyses also indicate that Iin14549 may play a role in lignan synthesis. These transcription factor families exhibited high expression in tetraploid I. indigotica, moderate expression in diploid I. indigotica, and low expression in I. tinctoria. The findings of this study can serve as a reference for improving the quality of I. indigotica and developing germplasms with high lignan content. Additionally, these results lay a foundation for the functional characterization of UGTs in lignan biosynthesis pathway.
菘蓝是一种含有具有重要经济价值的木脂素类化合物的植物物种。其根在治疗病毒方面起着关键作用，并具有抗肿瘤、抗炎、抗菌等生物活性。现在，菘蓝已被归入欧洲菘蓝。在本研究中，利用代谢组学和转录组学分析对二倍体菘蓝、四倍体菘蓝和欧洲菘蓝的根进行了分析。代谢组学分析检测到48种木脂素代谢物，包括鹅掌楸树脂醇A、弗拉迪诺醇A、丁香树脂醇、牛蒡子苷元、刺囊酸苷B和芝麻素作为特征性化合物，其余代谢物之间无显著差异。转录组学分析鉴定出41个差异表达的苯丙烷合酶基因，并进一步分析了不同样本间木脂素转录组图谱的变化。RT-qPCR分析还揭示了三个样本组中与木脂素生物合成途径相关的基因表达差异。转录因子分析表明，AP2-EREBP家族（Iin24319）、MYB家族（Iin2484,3）和WRKY家族（Iin08158）的表达模式与Iin14549相似。系统发育分析也表明Iin14549可能在木脂素合成中起作用。这些转录因子家族在四倍体菘蓝中高表达，在二倍体菘蓝中中等表达，在欧洲菘蓝中低表达。本研究结果可为提高菘蓝品质和培育高木脂素含量的种质资源提供参考。此外，这些结果为木脂素生物合成途径中UGTs的功能表征奠定了基础。
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