Xiao Ying, Feng Jingxian, Li Qing, Zhou Yangyun, Bu Qitao, Zhou Junhui, Tan Hexin, Yang Yingbo, Zhang Lei, Chen Wansheng
Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Department of Pharmacy, Changzheng Hospital, Naval Medical University (Second Military Medical University), Shanghai 200003, China.
Acta Pharm Sin B. 2020 Dec;10(12):2417-2432. doi: 10.1016/j.apsb.2019.12.020. Epub 2020 Jan 8.
Yield potential, pharmaceutical compounds production and stress tolerance capacity are 3 classes of traits that determine the quality of medicinal plants. The autotetraploid has greater yield, higher bioactive lignan accumulation and enhanced stress tolerance compared with its diploid progenitor. Here we show that the transcription factor WRKY34, with higher expression levels in tetraploid than in diploid , has large pleiotropic effects on an array of traits, including biomass growth rates, lignan biosynthesis, as well as salt and drought stress tolerance. Integrated analysis of transcriptome and metabolome profiling demonstrated that expression had far-reaching consequences on both primary and secondary metabolism, reprograming carbon flux towards phenylpropanoids, such as lignans and flavonoids. Transcript-metabolite correlation analysis was applied to construct the regulatory network of WRKY34 for lignan biosynthesis. One candidate target 4CL3, a key rate-limiting enzyme of lignan biosynthesis as indicated in our previous study, has been demonstrated to indeed be activated by WRKY34. Collectively, the results indicate that the differentially expressed WRKY34 has contributed significantly to the polyploidy vigor of , and manipulation of this gene will facilitate comprehensive improvements of herb.
产量潜力、药用化合物产量和胁迫耐受能力是决定药用植物品质的三类性状。与其二倍体祖先相比，同源四倍体具有更高的产量、更高的生物活性木脂素积累以及更强的胁迫耐受性。在此，我们表明转录因子WRKY34在四倍体中的表达水平高于二倍体，对一系列性状具有广泛的多效性影响，包括生物量生长速率、木脂素生物合成以及盐和干旱胁迫耐受性。转录组和代谢组谱的综合分析表明，WRKY34的表达对初级和次级代谢均产生了深远影响，将碳通量重新导向苯丙烷类化合物，如木脂素和黄酮类化合物。应用转录本-代谢物相关性分析构建了WRKY34调控木脂素生物合成的网络。一个候选靶标4CL3是我们之前研究中指出的木脂素生物合成的关键限速酶，已被证明确实被WRKY34激活。总体而言，结果表明差异表达的WRKY34对[植物名称]的多倍体活力有显著贡献，对该基因的操作将有助于全面改良[植物名称]药材。
