The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
World J Microbiol Biotechnol. 2023 Sep 4;39(11):298. doi: 10.1007/s11274-023-03736-8.
Saccharomyces cerevisiae is a health microorganism closely related to human life, especially in food and pharmaceutical industries. S. cerevisiae W303a and CEN.PK2-1C are two commonly used strains for synthetic biology-based natural product production. Yet, the metabolomic and transcriptomic differences between these two strains have not been compared. In this study, metabolomics and transcriptomics were applied to analyze the differential metabolites and differential expression genes (DEGs) between W303a and CEN.PK2-1C cultured in YPD and SD media. The growth rate of W303a in YPD medium was the lowest compared with other groups. When cultured in YPD medium, CEN.PK2-1C produced more phenylalanine than W303a; when cultured in SD medium, W303a produced more phospholipids than CEN.PK2-1C. Transcriptomic analysis revealed that 19 out of 22 genes in glycolysis pathway were expressed at higher levels in CEN.PK2-1C than that in W303a no matter which media were used, and three key genes related to phenylalanine biosynthesis including ARO9, ARO7 and PHA2 were up-regulated in CEN.PK2-1C compared with W303a when cultured in YPD medium, whereas seven DEGs associated with phospholipid biosynthesis were up-regulated in W303a compared with CEN.PK2-1C when cultured in SD medium. The high phenylalanine produced by CEN.PK2-1C and high phospholipids produced by W303a indicated that CEN.PK2-1C may be more suitable for synthesis of natural products with phenylalanine as precursor, whereas W303a may be more appropriate for synthesis of phospholipid metabolites. This finding provides primary information for strain selection between W303a and CEN.PK2-1C for synthetic biology-based natural product production.
酿酒酵母是一种与人类生活密切相关的有益微生物，尤其在食品和制药行业。酿酒酵母 W303a 和 CEN.PK2-1C 是两种常用于基于合成生物学的天然产物生产的常用菌株。然而，这两种菌株之间的代谢组学和转录组学差异尚未进行比较。在这项研究中，应用代谢组学和转录组学分析了 W303a 和 CEN.PK2-1C 在 YPD 和 SD 培养基中培养时的差异代谢物和差异表达基因（DEGs）。与其他组相比，W303a 在 YPD 培养基中的生长速度最慢。当在 YPD 培养基中培养时，CEN.PK2-1C 比 W303a 产生更多的苯丙氨酸；当在 SD 培养基中培养时，W303a 比 CEN.PK2-1C 产生更多的磷脂。转录组学分析表明，无论使用哪种培养基，22 个糖酵解途径中的 19 个基因在 CEN.PK2-1C 中的表达水平均高于 W303a，并且当在 YPD 培养基中培养时，与苯丙氨酸生物合成相关的三个关键基因 ARO9、ARO7 和 PHA2 在 CEN.PK2-1C 中上调，而当在 SD 培养基中培养时，与磷脂生物合成相关的七个 DEGs 在 W303a 中上调。CEN.PK2-1C 产生的高苯丙氨酸和 W303a 产生的高磷脂表明，CEN.PK2-1C 可能更适合合成以苯丙氨酸为前体的天然产物，而 W303a 可能更适合合成磷脂代谢物。这一发现为基于合成生物学的天然产物生产中 W303a 和 CEN.PK2-1C 之间的菌株选择提供了初步信息。
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