Tosar Juan Pablo
Functional Genomics Laboratory, Institut Pasteur de Montevideo, Uruguay.
Analytical Biochemistry Unit, Nuclear Research Center. School of Science, Universidad de la República, Uruguay.
Noncoding RNA Res. 2023 Aug 18;8(4):589-590. doi: 10.1016/j.ncrna.2023.08.007. eCollection 2023 Dec.
Several reports describing PIWI-interacting RNAs (piRNAs) in human cancer cells or in the bloodstream are affected by the presence of false positives in piRNA databases. A recent report suggested that piR-36249 regulates testicular cancer progression by engaging with DHX36 to regulate OAS2. However, piR-36249 is a tRNA-Cys 5' half capable of forming intermolecular G-quadruplexes. It is therefore expected that DHX36, a helicase with high affinity for DNA and RNA G-quadruplexes, was pulled down using piR-36249 mimicking probes. The suggestion of using piR-36249 as a therapeutic target for testicular cancer is therefore questionable, due to the consequences that tRNA inhibition could have on healthy cells.
几篇描述人类癌细胞或血液中PIWI相互作用RNA（piRNA）的报告受到piRNA数据库中假阳性存在的影响。最近的一份报告表明，piR-36249通过与DHX36结合来调节OAS2，从而调控睾丸癌的进展。然而，piR-36249是一种能够形成分子间G-四链体的tRNA-Cys 5' 半体。因此，预计使用模拟piR-36249的探针能够拉下对DNA和RNA G-四链体具有高亲和力的解旋酶DHX36。由于tRNA抑制可能对健康细胞产生的影响，因此将piR-36249用作睾丸癌治疗靶点的建议值得怀疑。
