Zhao Yaqi, Guo Huarong
Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ministry of Education, Ocean University of China, Qingdao 266003, China.
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
MethodsX. 2023 Aug 19;11:102335. doi: 10.1016/j.mex.2023.102335. eCollection 2023 Dec.
The development of a suitable shrimp cell medium is essential for achieving a long-term cell culture and finite cell line of shrimps routinely. In this study, we have successfully developed an optimal shrimp cell medium that can be used for long-term culture and continuous subculture of the hemolymph cells (or hemocytes) of greasyback shrimp , designated as MeH cells, by shrimp serum-based and supplements-based optimization of the basic and growth medium. In this article, we have focused on the details for the preparation of the optimal shrimp cell medium by diluting and mixing of various stock solutions as well as the methods for isolation and primary culture of MeH cells.•A novel shrimp cell growth medium is developed for long-term shrimp hemocytes culture.•The preparation method of shrimp cell growth medium is successfully established.•Obvious cell activity and proliferation potential of isolated shrimp cells can be maintained beyond 30 days.
开发合适的虾细胞培养基对于常规实现虾的长期细胞培养和有限细胞系至关重要。在本研究中，我们通过基于虾血清和基于补充剂对基础培养基和生长培养基进行优化，成功开发了一种最佳虾细胞培养基，可用于长期培养和连续传代培养斑节对虾的血淋巴细胞（或血细胞），命名为MeH细胞。在本文中，我们重点介绍了通过稀释和混合各种储备溶液制备最佳虾细胞培养基的细节以及MeH细胞的分离和原代培养方法。
•开发了一种用于虾血细胞长期培养的新型虾细胞生长培养基。
•成功建立了虾细胞生长培养基的制备方法。
•分离的虾细胞在30天以上可保持明显的细胞活性和增殖潜力。
