对联药通过影响阴道黏膜屏障来治疗外阴阴道假丝酵母菌病。
Extract of couplet medicines treats vulvovaginal candidiasis by affecting the vaginal mucosal barrier.
机构信息
Research Institute of Integrated Traditional Chinese & Western Medicine, Anhui Academy of Chinese Medicine, Hefei, 230012, China.
Department of Pathogenic Biology & Immunology, College of Integrated Chinese & Western Medicine, Anhui University of Chinese Medicine, Hefei, 230012, China.
出版信息
Future Microbiol. 2023 Aug;18:809-824. doi: 10.2217/fmb-2023-0033. Epub 2023 Sep 5.
This study investigated the inhibition of extract of couplet medicines (ESCC) on () and the effect of ESCC on the vaginal mucosal barrier . Susceptibility testing was performed with SC5314. A vulvovaginal candidiasis mouse model was successfully established. The plate method, Gram staining, hematoxylin and eosin staining and ELISA were used to detect relevant inflammatory indexes: IFN-γ, IL-1 and TNF-α. Quantitative real-time PCR and western blot were used to detect mucosal immune-related factors: MUC1, MUC4, DEFB1 and DEFB2. ESCC was able to inhibit the proliferative activity of , and it affected inflammation-related factors and indicators of vaginal mucosal immunity. ESCC showed potential value in the treatment of vulvovaginal candidiasis.
本研究考察了复方药提取物（ESCC）对（）的抑制作用及 ESCC 对阴道黏膜屏障的影响。采用 SC5314 进行药敏试验。成功建立了外阴阴道念珠菌病小鼠模型。采用平板法、革兰染色、苏木精-伊红染色和 ELISA 检测相关炎症指标：IFN-γ、IL-1 和 TNF-α。实时定量 PCR 和 Western blot 检测黏膜免疫相关因子：MUC1、MUC4、DEFB1 和 DEFB2。ESCC 能够抑制的增殖活性，影响炎症相关因子和阴道黏膜免疫指标。ESCC 在治疗外阴阴道念珠菌病方面具有潜在价值。
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