Jacobs W A, Leburg M W, Madaj E J
Anal Biochem. 1986 Aug 1;156(2):334-40. doi: 10.1016/0003-2697(86)90262-9.
The degradation of 1-alkylthio-2-alkylisoindoles, generated in situ by reaction of o-phthalaldehyde with primary alkylamines in the presence of selected thiols, was studied under conditions typical of those used in precolumn derivatization applications. In all cases, loss of isoindole followed pseudo first order kinetics and was strongly accelerated by excess o-phthalaldehyde in the reaction mixture. Reactivity toward o-phthalaldehyde was found to be influenced by a number of experimental parameters including thiol structure, thiol concentration, amine structure, solvent composition, and pH. In the absence of excess o-phthalaldehyde, the stability of all derivatives examined was excellent. These results provide a basis for optimization of reaction conditions for maximum derivative stability.
在柱前衍生化应用中常用的典型条件下，研究了由邻苯二甲醛与伯烷基胺在特定硫醇存在下原位生成的1-烷硫基-2-烷基异吲哚的降解情况。在所有情况下，异吲哚的损失遵循假一级动力学，并且反应混合物中过量的邻苯二甲醛会强烈加速其降解。发现对邻苯二甲醛的反应活性受多种实验参数影响，包括硫醇结构、硫醇浓度、胺结构、溶剂组成和pH值。在没有过量邻苯二甲醛的情况下，所有检测的衍生物稳定性都非常好。这些结果为优化反应条件以实现最大衍生物稳定性提供了依据。
