利用亮抑酶肽与牛胰蛋白酶的可逆结合来测定血浆中的亮抑酶肽。

Assay of plasma leupeptin using the reversible binding of leupeptin to bovine pancreatic trypsin.

作者信息

Yamashita K, Watanabe K, Takayama H, Mizuguchi S, Ishibashi M, Miyazaki H, Tanaka W, Umezawa H

出版信息

Anal Biochem. 1986 Aug 1;156(2):503-7. doi: 10.1016/0003-2697(86)90285-x.

Abstract

A competitive binding radioassay for leupeptin has been developed utilizing the reversible binding of leupeptin to bovine pancreatic trypsin. An ethanol precipitation step was introduced to separate trypsin-bound leupeptin from its free form. Advantages of this method are simplicity of the procedure and avoidance of the preparation of antiserum. The possible metabolites of leupeptin exhibit no significant inhibitory effect on leupeptin-trypsin binding in this system. This method was applied to the determination of plasma leupeptin levels in dogs after oral administration of the peptide.

摘要

利用亮抑酶肽与牛胰蛋白酶的可逆结合，开发了一种用于亮抑酶肽的竞争性结合放射分析方法。引入乙醇沉淀步骤以将与胰蛋白酶结合的亮抑酶肽与其游离形式分离。该方法的优点是操作简单且无需制备抗血清。在该系统中，亮抑酶肽的可能代谢产物对亮抑酶肽 - 胰蛋白酶结合无明显抑制作用。该方法用于测定犬口服该肽后血浆中亮抑酶肽的水平。