一种用于对等电聚焦凝胶上分离的蛋白质进行免疫印迹的方法。

A procedure for the immunoblotting of proteins separated on isoelectric focusing gels.

作者信息

Otey C A, Kalnoski M H, Bulinski J C

出版信息

Anal Biochem. 1986 Aug 15;157(1):71-6. doi: 10.1016/0003-2697(86)90197-1.

Abstract

A method has been devised for performing Western blot assays on proteins resolved by isoelectric focusing. Electrophoretic transfer of proteins directly from isoelectric focusing (IEF) tube gels to nitrocellulose sheets allowed their immunoassay without conventional second dimension SDS gel electrophoresis. The same method can also be used for IEF slab gels. For the immunostaining of nonmuscle actin isoforms in extracts of cultured cells, the resolution of this technique was much improved over that of Western blots of two-dimensional gels.

摘要

已经设计出一种方法，用于对通过等电聚焦分离的蛋白质进行蛋白质免疫印迹分析。蛋白质从等电聚焦（IEF）管凝胶直接电泳转移至硝酸纤维素膜上，无需传统的二维SDS凝胶电泳即可进行免疫分析。相同的方法也可用于IEF平板凝胶。对于培养细胞提取物中非肌肉肌动蛋白异构体的免疫染色，该技术的分辨率比二维凝胶的蛋白质免疫印迹法有了很大提高。