Xing Yanan, Cao Yilin, Ma Yanan, Wang Fu, Xin Shijie, Zhu Wenying
Qingdao Agricultural University, Qingdao, China.
Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao, China.
Front Plant Sci. 2023 Aug 21;14:1208675. doi: 10.3389/fpls.2023.1208675. eCollection 2023.
A total of 151 recombinant inbred lines (RILs) were derived from the cross between ' L. ' (HW) and a cultivated Northern Chinese inbred line 'XinTaiMiCi' (XTMC). We used resequencing to construct the genetic map and analyze the genetic background of RIL population, and combined with the phenotypes of RIL population and the analysis of RNA-seq data, we located the major loci controlling the fruit length of cucumber and related analysis. A genetic map containing 600 bin markers was constructed via re-sequencing. Based on the phenotype data collected in two different seasons (spring 2021 and autumn 2022), the major quantitative trait loci (QTLs) controlling cucumber fruit length were located and their transcriptomic analysis carried out. The results revealed three QTLs (, , and ) detected repeatedly in the two seasons, of which was the dominant QTL. From the functional annotation of corresponding genes there, we discovered the gene encoding an auxin efflux carrier family protein. The expression of that gene was significantly lower in XTMC and the long-fruit RIL lines than in HW and the short-fruit RIL lines; hence, we speculated the gene could be negatively correlated with the fruit length of cucumber. Transcriptomic analysis showed that 259 differentially expressed genes (DEGs) were enriched in the plant hormone signal transduction pathway. In addition, among those DEGs, 509 transcription factors were detected, these distributed in several transcription factor gene families, such as bHLH, AP2/ErF -ERF, C2H2, and NAC. Therefore, we concluded that the major gene controlling the fruit length of cucumber is located in the interval of , whose gene may be involved in the negative regulation of fruit length. Further, genes related to plant hormone signal transduction and several transcription factors were also found involved in the regulation of cucumber fruit length. Our results provide a reference for the fine mapping of major genes and analyzing the mechanism of cucumber fruit length.
总共151个重组自交系（RILs）源自“L.”（HW）与一个中国北方栽培自交系“新泰密刺”（XTMC）的杂交。我们利用重测序构建遗传图谱并分析RIL群体的遗传背景，并结合RIL群体的表型和RNA-seq数据的分析，定位了控制黄瓜果实长度的主要位点并进行了相关分析。通过重测序构建了一个包含600个bin标记的遗传图谱。基于在两个不同季节（2021年春季和2022年秋季）收集的表型数据，定位了控制黄瓜果实长度的主要数量性状位点（QTLs）并进行了转录组分析。结果揭示了在两个季节中反复检测到的三个QTLs（、和），其中是主要QTL。从那里相应基因的功能注释中，我们发现基因编码一种生长素外流载体家族蛋白。该基因在XTMC和长果RIL系中的表达明显低于HW和短果RIL系；因此，我们推测该基因可能与黄瓜果实长度呈负相关。转录组分析表明，259个差异表达基因（DEGs）在植物激素信号转导途径中富集。此外，在这些DEGs中，检测到509个转录因子，它们分布在几个转录因子基因家族中，如bHLH、AP2/ErF-ERF、C2H2和NAC。因此，我们得出结论，控制黄瓜果实长度的主要基因位于区间内，其基因可能参与果实长度的负调控。此外，还发现与植物激素信号转导相关的基因和几个转录因子也参与黄瓜果实长度的调控。我们的结果为主要基因的精细定位和分析黄瓜果实长度的机制提供了参考。
