Sport and Specialised Analytical Services, LGC, Fordham, UK.
British Horseracing Authority, London, UK.
Drug Test Anal. 2024 May;16(5):498-503. doi: 10.1002/dta.3569. Epub 2023 Sep 6.
Gene doping in horses is a threat to the fairness in sport and has serious implications for animal welfare. To investigate the effect of long-term storage on the detection of AAV in plasma and whole blood, samples from an administration study using an adeno-associated virus serotype 6 expressing green fluorescence protein (AAV6-GFP) were stored at -20°C for 8 months before analysis. The AAV vector was detected in stored plasma samples, following the same detection profile as the fresh plasma samples. The stored blood showed lower overall DNA detection but followed the same detection profile as the plasma samples. This study provides confidence that re-analysing plasma samples and/or analysing a frozen 'B' sample with different matrix such as whole blood after prolonged storage will still result in the detection of gene doping material.
马科基因兴奋剂是对体育公平性的威胁，也对动物福利有严重影响。为了研究长期储存对检测血液中腺相关病毒（AAV）的影响，本研究使用表达绿色荧光蛋白的腺相关病毒血清型 6（AAV6-GFP）进行了一项给药研究，在分析前将采集的样本储存在-20°C 下 8 个月。储存的血浆样本中可检测到 AAV 载体，与新鲜血浆样本的检测模式相同。储存的血液总体 DNA 检测水平较低，但与血浆样本的检测模式相同。本研究表明，在长时间储存后重新分析血浆样本和/或使用不同基质（如全血）分析冷冻的“B”样本，仍将检测到基因兴奋剂物质。
