Houshyar Mohammad, Saki Ali Asghar, Alikhani Mohammad Yousef, Bedford Michael Richard, Soleimani Meysam, Kamarehei Farideh
Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Poult Sci. 2023 Nov;102(11):103014. doi: 10.1016/j.psj.2023.103014. Epub 2023 Aug 9.
This study aimed to evaluate the effects of a laboratory 3-phytase (the expression of the phyK gene, Lab-Phy) and a commercial 6-phytase (Quantum Blue 40 P, Com-Phy) alone and in combination (corn-soy-based diets) in broilers. A total of 400, day-old Ross 308 male broilers were randomly assigned to 5 treatments with 10 replicate cages (8 chicks/cage) for a 14-day trial. Experimental treatments included the positive control (0.95% Ca and 0.48% nonphytate phosphorus (nPP), PC), negative control (0.90% Ca and 0.22% nPP, NC), and NC which was supplemented with Lab-Phy 250 FTU/kg and Com-Phy 250 FTU/kg alone or in combination of Lab-Phy 125 FTU/kg and Com-Phy 125 FTU/kg. The inclusion of Lab-Phy in the NC diet significantly improved the P and Ca content in the tibia compared to the NC group. Moreover, the inclusion of Com-Phy alone and in combination with Lab-Phy in the NC diet significantly increased the P and Ca content in the tibia compared to the Lab-Phy. The mRNA expression of NaPi-IIb was upregulated in the duodenum by the reduction of nPP and downregulated by the inclusion of any phytase, whereas other nutrient transporters were not influenced by the reduction of nPP or the addition of phytase in the small intestine mucosa. Broilers receiving the NC diet obtained the lowest body weight (BW) and body weight gain (BWG) at 8 to 14 and 1 to 14 d of age. The NC group showed the lowest villi height and surface area, Newcastle disease (ND) antibody titer, and digestibility of nutrients compared to the PC group at 14 d of age. Supplementing the NC diet with the Lab-Phy and Com-Phy individually, or in combination tended to improve BW, BWG, tibia characteristics, villi characteristics, ND, and retained CP and P, and apparent ileal digestibility of CP, P, methionine, and threonine. The present research indicated that the studied traits by the combination of phytases were slightly better than the average of the 2 individually, suggesting there might be some value in combining the laboratory and commercial phytases.
本研究旨在评估实验室3-植酸酶（phyK基因表达产物，实验室植酸酶）和商业6-植酸酶（量子蓝40P，商品植酸酶）单独及联合使用（基于玉米-豆粕的日粮）对肉鸡的影响。总共400只1日龄罗斯308雄性肉鸡被随机分配到5种处理组，每组10个重复笼（每个笼子8只鸡），进行为期14天的试验。试验处理包括阳性对照（0.95%钙和0.48%非植酸磷（nPP），PC）、阴性对照（0.90%钙和0.22% nPP，NC），以及在NC基础上单独添加250 FTU/kg实验室植酸酶和250 FTU/kg商品植酸酶，或联合添加125 FTU/kg实验室植酸酶和125 FTU/kg商品植酸酶。与NC组相比，在NC日粮中添加实验室植酸酶显著提高了胫骨中的磷和钙含量。此外，与单独添加实验室植酸酶相比，在NC日粮中单独添加商品植酸酶以及与实验室植酸酶联合添加均显著提高了胫骨中的磷和钙含量。十二指肠中NaPi-IIb的mRNA表达因nPP减少而上调，因添加任何植酸酶而下调，而其他营养物质转运体不受小肠黏膜中nPP减少或植酸酶添加的影响。在8至14日龄和1至14日龄时，接受NC日粮的肉鸡体重（BW）和体重增加（BWG）最低。与14日龄时的PC组相比，NC组的绒毛高度和表面积、新城疫（ND）抗体效价以及营养物质消化率最低。在NC日粮中单独或联合添加实验室植酸酶和商品植酸酶倾向于改善BW、BWG、胫骨特征、绒毛特征、ND，以及粗蛋白和磷的保留量，以及粗蛋白、磷、蛋氨酸和苏氨酸的回肠表观消化率。本研究表明，植酸酶联合使用对所研究性状的改善略优于二者单独使用的平均值，这表明将实验室植酸酶和商业植酸酶联合使用可能具有一定价值。
