Belayew A, Bellefroid E, Berwaer M, Dumoulin M, Lambert C, Lemaitre-Wathy C, Mathy-Hartert M, Morin A, Pasleau F, Scippo M L
Ann Endocrinol (Paris). 1986;47(1):7-10.
We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and placental lactogen (hCS-B). We have cloned these genes and are searching within their sequences for in vitro binding sites of the human glucocorticoid receptor on the hGH-N and hCS-B genes; the in vivo activity of such DNA sequences by assaying hybrid gene expression in transfected cells; in vivo "enhancer" activity of different hPRL gene fragments linked to a marker gene and transfected in cultured cells.
我们正在研究负责真核基因表达调控的核苷酸序列。我们的测试系统包括编码催乳素（hPRL）、生长激素（hGH-N）和胎盘催乳素（hCS-B）的人类基因。我们已经克隆了这些基因，并在其序列中寻找人类糖皮质激素受体在hGH-N和hCS-B基因上的体外结合位点；通过检测转染细胞中的杂交基因表达来研究此类DNA序列的体内活性；将不同的hPRL基因片段与标记基因相连并转染到培养细胞中，研究其体内“增强子”活性。
