Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand.
Int J Biol Macromol. 2023 Dec 31;253(Pt 2):126712. doi: 10.1016/j.ijbiomac.2023.126712. Epub 2023 Sep 5.
Bacterial cellulose (BC) is a biomaterial being investigated for a range of applications. Herein, BC films derived from nata de coco pieces are reinforced by two-dimensional molybdenum disulfide (MoS) and helical carbon nanotubes (HCNTs) to enhance their tensile mechanical properties, and the biocompatibility of the BC composite films is demonstrated. A simple preparation is presented using a kitchen blender to disperse and blend the BC fibers and additives in a common fabrication medium, followed by vacuum filtration. The mechanical properties of the BC/MoS/HCNTs composite films are enhanced due to the synergistic effect of MoS and HCNTs embedded in the BC films. The MoS/HCNTs binary additive (1 phr) is capable of increasing the strength and Young's modulus by 148 % and 333 %, respectively, relative to the BC films. The cell cytotoxicity of the BC/MoS/HCNTs films was assessed using an MTT assay. The composite films are biocompatible with a cell viability of L929 fibroblast cells >70 %, coupled with observations of direct cell attachment on the films. The composite films also exhibited good performance in absorbing and releasing gentamicin antibiotics to inhibit the growth of Escherichia coli and Staphylococcus aureus. The BC/MoS/HCNTs films are thus potential BC-based candidates as biocompatible robust antibiotic carriers.
细菌纤维素（BC）是一种正在被研究用于多种应用的生物材料。在此，我们使用椰果衍生的 BC 薄膜，通过二维二硫化钼（MoS）和螺旋状碳纳米管（HCNT）进行增强，以提高其拉伸力学性能，并展示了 BC 复合薄膜的生物相容性。我们采用简单的制备方法，使用厨房搅拌机在常见的制备介质中分散和混合 BC 纤维和添加剂，然后进行真空过滤。由于嵌入 BC 薄膜中的 MoS 和 HCNT 的协同作用，BC/MoS/HCNTs 复合薄膜的机械性能得到了增强。MoS/HCNTs 二元添加剂（1 phr）相对于 BC 薄膜能够分别将强度和杨氏模量提高 148%和 333%。我们通过 MTT 分析评估了 BC/MoS/HCNTs 薄膜的细胞毒性。复合薄膜具有良好的生物相容性，L929 成纤维细胞的存活率>70%，并且观察到细胞直接附着在薄膜上。复合薄膜还表现出良好的吸收和释放庆大霉素抗生素的性能，以抑制大肠杆菌和金黄色葡萄球菌的生长。因此，BC/MoS/HCNTs 薄膜是一种有潜力的基于 BC 的生物相容性、强韧的抗生素载体候选材料。
