来自墨西哥杜兰戈的一条墨西哥黑尾响尾蛇（Crotalus molossus nigrescens）毒液成分的个体发育变化。

Ontogenetic change in the venom composition of one Mexican black-tailed rattlesnake (Crotalus molossus nigrescens) from Durango, Mexico.

作者信息

Borja Miguel, Neri-Castro Edgar, Gutiérrez-Martínez Arelí, Bledsoe Richard, Zarzosa Vanessa, Rodriguez-López Bruno, Strickland Jason L, Becerra-López Jorge, Valenzuela-Ceballos Sara, Parkinson Christopher L, Alagón Alejandro, Castañeda-Gaytán Gamaliel

机构信息

Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Av. Universidad s/n. Fracc. Filadelfia, C.P. 35010, Gómez Palacio, Dgo., Mexico.

Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Av. Universidad s/n. Fracc. Filadelfia, C.P. 35010, Gómez Palacio, Dgo., Mexico; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Chamilpa, C.P. 62210, Cuernavaca, Mor., Mexico.

出版信息

Toxicon. 2023 Oct;234:107280. doi: 10.1016/j.toxicon.2023.107280. Epub 2023 Sep 4.

DOI:10.1016/j.toxicon.2023.107280

PMID:37673344

Abstract

To corroborate the ontogenetic shift in the venom composition of the Mexican Black-tailed Rattlesnake (Crotalus molossus nigrescens) previously reported through the census approach, we evaluated the shift in the protein profile, lethality, and proteolytic and phospholipase activities of four venom samples obtained in 2015, 2018, 2019, and 2021 from one C. m. nigrescens individual (CMN06) collected in Durango, Mexico. We demonstrated that the venom of C. m. nigrescens changed from a myotoxin-rich venom to a phospholipase A and snake venom metalloproteinase-rich venom. Additionally, the proteolytic and phospholipase activities increased with age, but the lethality decreased approximately three times.

摘要

为了证实先前通过普查方法报道的墨西哥黑尾响尾蛇（Crotalus molossus nigrescens）毒液成分的个体发育转变，我们评估了2015年、2018年、2019年和2021年从墨西哥杜兰戈采集的一条黑尾响尾蛇个体（CMN06）的四个毒液样本在蛋白质谱、致死性、蛋白水解活性和磷脂酶活性方面的转变。我们证明，黑尾响尾蛇的毒液从富含肌毒素的毒液转变为富含磷脂酶A和蛇毒金属蛋白酶的毒液。此外，蛋白水解活性和磷脂酶活性随年龄增长而增加，但致死性下降了约三倍。