Tian Yuan, Liu Yang, Uwaremwe Constantine, Zhao Xia, Yue Liang, Zhou Qin, Wang Yun, Tran Lam-Son Phan, Li Weiqiang, Chen Gaofeng, Sha Yuexia, Wang Ruoyu
Gansu Gaolan Field Scientific Observation and Research Station for Agricultural Ecosystem, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, People's Republic of China.
State Key Laboratory of Grassland Agro-Ecosystems, Center for Grassland Microbiome, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou, 730000, People's Republic of China.
Plant Cell Rep. 2023 Nov;42(11):1757-1776. doi: 10.1007/s00299-023-03060-3. Epub 2023 Sep 7.
The novel interkingdom PGPM consortia enhanced the ability of plant growth promotion and disease resistance, which would be beneficial to improve plant growth in sustainable agriculture through engineering microbiome. Plant growth-promoting microbes (PGPMs) play important roles in promoting plant growth and bio-controlling of pathogens. Much information reveals that the plant growth-promoting ability of individual PGPM affects plant growth. However, the effects of the PGPM consortia properties on plant growth remain largely unexplored. Here, we characterized three new PGPM strains including Rhodotorula graminis JJ10.1 (termed as J), Pseudomonas psychrotolerans YY7 (termed as Y) and P. chlororaphis T8 (termed as T), and assessed their effects in combination with Bacillus amyloliquefaciens FZB42 (termed as F) on plant growth promotion and disease prevention in Arabidopsis thaliana and tomato (Solanum lycopersicum) plants by investigating morphological changes, whole-genome sequencing and plant growth promoting (PGP) characterization. Results revealed that the three new strains R. graminis JJ10.1, P. psychrotolerans YY7 and P. chlororaphis T8 had the potential for being combined with B. amyloliquefaciens FZB42 to form interkingdom PGPM consortia. The combinations of R. graminis JJ10.1, B. amyloliquefaciens FZB42, and P. psychrotolerans YY7, i. e. JF and JYF, exhibited the strongest ability of synergetic biofilm production. Furthermore, the growth-promotion abilities of the consortia were significantly enhanced compared with those of individual strains under both inoculation and volatile organic compounds (VOCs) treatment. Importantly, the consortia showed stronger abilities of in planta disease prevention than individual strains. Findings of our study may provide future guidance for engineering the minimal microbiome communities to improve plant growth and/or disease resistance in sustainable agriculture.
新型跨界植物促生微生物联合体增强了植物生长促进和抗病能力，这将有利于通过构建微生物群落来改善可持续农业中的植物生长。植物促生微生物（PGPMs）在促进植物生长和对病原体进行生物防治方面发挥着重要作用。大量信息表明，单个PGPM的植物促生能力会影响植物生长。然而，PGPM联合体的特性对植物生长的影响在很大程度上仍未得到探索。在此，我们鉴定了三株新的PGPM菌株，包括禾本科红酵母JJ10.1（称为J）、耐冷假单胞菌YY7（称为Y）和绿针假单胞菌T8（称为T），并通过研究形态变化、全基因组测序和植物促生（PGP）特性，评估了它们与解淀粉芽孢杆菌FZB42（称为F）组合对拟南芥和番茄植株生长促进和疾病预防的影响。结果表明，三株新菌株禾本科红酵母JJ10.1、耐冷假单胞菌YY7和绿针假单胞菌T8有潜力与解淀粉芽孢杆菌FZB42组合形成跨界PGPM联合体。禾本科红酵母JJ10.1、解淀粉芽孢杆菌FZB42和耐冷假单胞菌YY7的组合，即JF和JYF，表现出最强的协同生物膜形成能力。此外，在接种和挥发性有机化合物（VOCs）处理下，联合体的促生长能力与单个菌株相比均显著增强。重要的是，联合体在植物体内的防病能力比单个菌株更强。我们的研究结果可能为构建最小微生物群落以改善可持续农业中的植物生长和/或抗病性提供未来指导。
